Amino Acids

, Volume 37, Issue 1, pp 169–175 | Cite as

Dietary l-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs

  • Bie Tan
  • Yulong Yin
  • Zhiqiang Liu
  • Xinguo Li
  • Haijun Xu
  • Xiangfeng Kong
  • Ruilin Huang
  • Wenjie Tang
  • Izuru Shinzato
  • Stephen B. Smith
  • Guoyao Wu
Original Article

Abstract

Obesity in humans is a major public health crisis worldwide. In addition, livestock species exhibit excessive subcutaneous fat at market weight. However, there are currently few means of reducing adiposity in mammals. This study was conducted with a swine model to test the hypothesis that dietary l-arginine supplementation may increase muscle gain and decrease fat deposition. Twenty-four 110-day-old barrows were assigned randomly into two treatments, representing supplementation with 1.0% l-arginine or 2.05% l-alanine (isonitrogenous control) to a corn- and soybean meal-based diet. Growth performance was measured based on weight gain and food intake. After a 60-day period of supplementation, carcass and muscle composition were measured. Serum triglyceride concentration was 20% lower (P < 0.01) but glucagon level was 36% greater (P < 0.05) in arginine-supplemented than in control pigs. Compared with the control, arginine supplementation increased (P < 0.05) body weight gain by 6.5% and carcass skeletal-muscle content by 5.5%, while decreasing (P < 0.01) carcass fat content by 11%. The arginine treatment enhanced (P < 0.05) longissimus dorsi muscle protein, glycogen, and fat contents by 4.8, 42, and 70%, respectively, as well as muscle pH at 45 min post-mortem by 0.32, while reducing muscle lactate content by 37%. These results support our hypothesis that dietary arginine supplementation beneficially promotes muscle gain and reduces body fat accretion in growing-finishing pigs. The findings have a positive impact on development of novel therapeutics to treat human obesity and enhance swine lean-tissue growth.

Keywords

Arginine Growth Muscle Fat Meat quality 

Abbreviations

ACC

Acetyl-CoA carboxylase

ADG

Average daily gain

DM

Dry matter

LD

Longissimus dorsi

NO

Nitric oxide

SM

Semitendinosus

References

  1. AOAC (1996) Official methods of analysis. In: Cunniff P (ed) Association of Official Analytical Chemists (AOAC) International, 16th edn. AOAC, GaithersburgGoogle Scholar
  2. Azain MJ (2004) Role of fatty acids in adipocyte growth and development. J Anim Sci 82:916–924PubMedGoogle Scholar
  3. Bendall JK, Swatland HJ (1988) A review of the relationship of pH with physical aspects of pork quality. Meat Sci 24:85–96CrossRefGoogle Scholar
  4. Bergen W, Mersmann HJ (2005) Comparative aspects of lipid metabolism: impact on contemporary research and use of animal models. J Nutr 135:2499–2502PubMedGoogle Scholar
  5. Chung KY, Choi CB, Kawachi H, Yano H, Smith SB (2005) Trans-10, cis-12 conjugated linoleic acid antagonizes arginine-promoted differentiation of bovine preadipocytes. Adipocytes 2:93–100Google Scholar
  6. Deng D, Yin YL, Chu WY, Yao K, Li TJ, Huang RL, Liu ZQ, Zhang JS, Wu G (2008) Impaired translation initiation activation and reduced protein synthesis in weaned piglets fed a low-protein diet. J Nutr Biochem (in press)Google Scholar
  7. Ding ST, Schinckel AP, Weber TE, Mersmann HJ (2000) Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations. J Anim Sci 78:2127–2134PubMedGoogle Scholar
  8. Etherton TD, Walton PE (1986) Hormonal and metabolic regulation of lipid metabolism in domestic livestock. J Anim Sci 63:76–88Google Scholar
  9. Frank J, Escobar J, Nguyen HV, Jobgen SC, Jobgen WS, Davis TA, Wu G (2007) Oral N-carbamylglutamate supplementation increases protein synthesis in skeletal muscle of pigets. J Nutr 137:315–319PubMedGoogle Scholar
  10. Fu WJ, Haynes TE, Kohli R, Hu J, Shi W, Spencer TE, Carroll RJ, Meininger CJ, Wu G (2005) Dietary l-arginine supplementation reduces fat mass in Zucker diabetic fatty rats. J Nutr 135:714–721PubMedGoogle Scholar
  11. GB8467-87 (1988) Performance measurement technology regulations for Chinese lean pig. China Standard Press, BeijingGoogle Scholar
  12. Henckel P, Karlsson A, Oksbjerg N, Petersen JS (2000) Control of post mortem pH decrease in pig muscles: experimental design and testing of animal models. Meat Sci 55:131–138CrossRefGoogle Scholar
  13. Hill JO, Peters JC, Catenacci VA, Wyatt HR (2008) International strategies to address obesity. Obesity Rev 9(Suppl 1):41–47CrossRefGoogle Scholar
  14. Honikel KO (1998) Reference methods for the assessment of physical characteristics of meat. Meat Sci 49:447–457CrossRefGoogle Scholar
  15. Hu CA, Khalil S, Zhaorigetu S, Liu Z, Tyler M, Wan G, Valle D (2008) Human Δ1 pyrroline-5-carboxylate synthase: function and regulation. Amino Acids. doi:10.1007/s00726-008-0075-0
  16. Jobgen WS, Fried SK, Fu WJ, Meininger CJ, Wu G (2006) Regulatory role for the arginine-nitric oxide pathway in metabolism of energy substrates. J Nutr Biochem 17:571–588PubMedCrossRefGoogle Scholar
  17. Kim SW, Wu G (2004) Dietary arginine supplementation enhances the growth of milk-fed young pigs. J Nutr 134:625–630PubMedGoogle Scholar
  18. Lucotti P, Setola E, Monti LD, Galluccio E, Costa S, Sandoli EP, Fermo I, Rabaiotti G, Gatti R, Piatti P (2006) Beneficial effect of a long-term oral l-arginine treatment added to a hypocaloric diet and exercise training program in obese, insulin-resistant type 2 diabetic patients. Am J Physiol Endocrinol Metab 291:E906–E912PubMedCrossRefGoogle Scholar
  19. Mersmann HJ, Smith SB (2005) Development of white adipose tissue lipid metabolism. In: Burrin DG, Mersmann HJ (eds) Biology of metabolism in growing animals. Elsevier, Oxford, pp 275–302Google Scholar
  20. Montanez R, Sanchez-Jimenez F, Aldana-Montes JF, Medina MA (2007) Polyamines: metabolism to systems biology and beyond. Amino Acids 33:283–289PubMedCrossRefGoogle Scholar
  21. Munday MR (2002) Regulation of mammalian acetyl-CoA carboxylase. Biochem Soc Trans 30:1059–1064PubMedCrossRefGoogle Scholar
  22. Nikolic J, Stojanovic I, Pavlovic R, Sokolovic D, Bjelakovic G, Beninati S (2007) The role of l-arginine in toxic liver failure: interrelation of arginase, polyamine catabolic enzymes and nitric oxide synthase. Amino Acids 32(1):127–131PubMedCrossRefGoogle Scholar
  23. Nisoli E, Clementi E, Paolucci C, Cozzi V, Tonello C, Sciorati C, Bracale R, Valerio A, Francolini M, Moncada S, Carruba MO (2003) Mitochondrial biogenesis in mammals: the role of endogenous nitric oxide. Science 299:896–899PubMedCrossRefGoogle Scholar
  24. NRC (1998) Nutrient requirements of swine. National Academy Press, Washington DCGoogle Scholar
  25. Ou DY, Li DF, Cao YH, Li XL, Yin JD, Qiao SY, Wu G (2007) Dietary supplementation with zinc oxide decreases expression of the stem cell factor in the small intestine of weanling pigs. J Nutr Biochem 18:820–826PubMedCrossRefGoogle Scholar
  26. Rincker PJ, Killefer J, Ellis M, Brewer MS, McKeith FK (2008) Intramuscular fat content has little influence on the eating quality of fresh pork loin chops. J Anim Sci 86:730–737PubMedCrossRefGoogle Scholar
  27. Rosenvold K, Petersen JS, Lærke HN, Jensen SK, Therkildsen M, Karlsson AH, Møller HS, Andersen HJ (2001) Muscle glycogen stores and meat quality as affected by strategic finishing feeding of slaughter pigs. J Anim Sci 79:382–391PubMedGoogle Scholar
  28. Smith SB, Mersmann HJ, Smith EO, Britain KG (1999) Stearoyl-coenzyme A desaturase gene expression during growth in adipose tissue from obese and crossbred pigs. J Anim Sci 77:1710–1716PubMedGoogle Scholar
  29. Tan BE, Li XG, Kong XF, Huang RL, Ruan Z, Deng ZY, Xie MY, Shinzato I, Yin YL, Wu G (2008) Dietary l-arginine supplementation enhances the immune status in early-weaned piglets. Amino Acids. doi:10.1007/s00726-008-0155-1 Google Scholar
  30. Wilborn BS, Kerth CR, Owsley WF, Jones WR, Frobish LT (2004) Improving pork quality by feeding supranutritional concentrations of vitamin D3. J Anim Sci 82:218–224PubMedGoogle Scholar
  31. Wu G, Bazer FW, Davis TA, Jaeger LA, Johnson GA, Kim SW, Knabe DA, Meininger CJ, Spencer TE, Yin YL (2007a) Important roles for the arginine family of amino acids in swine nutrition and production. Livest Sci 112:8–22CrossRefGoogle Scholar
  32. Wu G, Bazer FW, Cudd TA, Jobgen WS, Kim SW, Lassala A, Li P, Matis JH, Meininger CJ, Spencer TE (2007b) Pharmacokinetics and safety of arginine supplementation in animals. J Nutr 137:1673S–1680SPubMedGoogle Scholar
  33. Wu G, Collins JK, Perkins-Veazie P, Siddiq M, Dolan KD, Kelly KA, Heaps CL, Meininger CJ (2007c) Dietary supplementation with watermelon pomace juice enhances arginine availability and ameliorates the metabolic syndrome in Zucker diabetic fatty rats. J Nutr 137:2680–2685PubMedGoogle Scholar
  34. Wu G, Davis PK, Flynn NE, Knabe DA, Davidson JT (1997) Endogenous synthesis of arginine plays an important role in maintaining arginine homeostasis in postweaning growing pigs. J Nutr 127:2342–2349PubMedGoogle Scholar
  35. Wu G, Meininger CJ (2000) Arginine nutrition and cardiovascular function. J Nutr 130:2626–2629PubMedGoogle Scholar
  36. Wu G, Morris SM Jr (1998) Arginine metabolism: nitric oxide and beyond. Biochem J 336:1–17PubMedGoogle Scholar
  37. Yan HY, Aziz E, Shillabeer G, Wong A, Shanghavi D, Kermouni A, Abdel-Hafez M, Lau DCW (2002) Nitric oxide promotes differentiation of rat white preadipocytes in culture. J Lipid Res 43:2123–2129PubMedCrossRefGoogle Scholar
  38. Yao K, Deng D, Liu ZQ, Li TJ, Huang RL, Chu WY, Tan BE, Wang W, Wu G, Yin YL (2008) Dietary arginine supplementation increases intracellular mTOR signaling activity in skeletal muscle of neonatal pigs. J Nutr 138:867–872PubMedGoogle Scholar
  39. Yin YL, Deng ZY, Huang RL, Li TJ, Zhong HY (2004) The effect of arabinoxylanase and protease supplementation on nutritional value of diets containing wheat bran or rice bran in growing pig. J Anim Feed Sci 13:445–461Google Scholar
  40. Zou CH, Shao JH (2008) Role of adipocytokines in obesity-associated insulin resistance. J Nutr Biochem 19:277–286PubMedCrossRefGoogle Scholar
  41. Zou MH, Kirkpatrick SS, Davis BJ, Nelson JS, Wiles WG, Schlattner U, Neumann D, Brownlee M, Freeman MB, Goldman MH (2004) Activation of the AMP-activated protein kinase by the anti-diabetic drug metformin in vivo. Role of mitochondrial reactive nitrogen species. J Biol Chem 279:43940–43951PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Bie Tan
    • 1
    • 2
  • Yulong Yin
    • 1
  • Zhiqiang Liu
    • 1
    • 2
  • Xinguo Li
    • 3
  • Haijun Xu
    • 1
    • 2
  • Xiangfeng Kong
    • 1
  • Ruilin Huang
    • 1
  • Wenjie Tang
    • 1
    • 2
  • Izuru Shinzato
    • 4
  • Stephen B. Smith
    • 5
  • Guoyao Wu
    • 1
    • 5
  1. 1.Laboratory of Animal Nutrition and Human Health and Key Laboratory of Agro-ecology, Institute of Subtropical AgricultureThe Chinese Academy of SciencesChangshaChina
  2. 2.The Graduate School of The Chinese Academy of SciencesBeijingChina
  3. 3.Hunan Institute of Animal Husbandry and Veterinary MedicineChangshaChina
  4. 4.Ajinomoto Co., Inc.TokyoJapan
  5. 5.Department of Animal ScienceTexas A&M UniversityCollege StationUSA

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