Abstract
Synthesis and properties of a new fluorescent/fluorogenic substrate Ac-DEVD-AMAC for caspase-3 are reported. The substrate is obtained by conventional Fmoc-based solid phase peptide synthesis and its properties are investigated with regard to fluorescence, sensitivity, applicability and kinetic constants. A non-traditional approach to assay the proteases activity using 2-aminoacridone labeled peptides is proposed. This approach utilizes the decrease of fluorescence intensity of a sample as a measure for the enzyme activity.
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This study was financially supported by The National Science Fund of Bulgaria (Grand BYX-10/2005).
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Lozanov, V., Ivanov, I.P., Benkova, B. et al. Peptide substrate for caspase-3 with 2-aminoacridone as reporting group. Amino Acids 36, 581–586 (2009). https://doi.org/10.1007/s00726-008-0136-4
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DOI: https://doi.org/10.1007/s00726-008-0136-4