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Localization and expression of serine racemase in Arabidopsis thaliana

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Abstract

Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with β-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by l- and d-serine, light irradiation, biotic and abiotic stresses.

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Acknowledgments

This research was supported in part by the Ohara Foundation in Kurashiki, Japan.

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Authors and Affiliations

  1. Group of Cytomolecular Biochemistry, Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama, 710-0046, Japan

    Manabu Sugimoto

  2. Group of Environmental Signaling Reaction, Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, Japan

    Wataru Sakamoto

  3. Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

    Yoshiyuki Fujitani

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  1. Manabu Sugimoto
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  2. Wataru Sakamoto
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  3. Yoshiyuki Fujitani
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Correspondence to Manabu Sugimoto.

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Sugimoto, M., Sakamoto, W. & Fujitani, Y. Localization and expression of serine racemase in Arabidopsis thaliana . Amino Acids 36, 587–590 (2009). https://doi.org/10.1007/s00726-008-0112-z

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  • DOI: https://doi.org/10.1007/s00726-008-0112-z

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