Skip to main content
Log in

Serum amino acid profile in patients with acute pancreatitis

  • Published:
Amino Acids Aims and scope Submit manuscript

Summary.

Patients in the early phase of acute pancreatitis (AP) have reduced serum levels of arginine and citrulline. This may be of patho-biological importance, since arginine is the substrate for nitric oxide, which in turn is involved in normal pancreatic physiology and in the inflammatory process. Serum amino acid spectrum was measured daily for five days and after recovery six weeks later in 19 patients admitted to the hospital for acute pancreatitis. These patients had abnormal levels of most amino acids including arginine, citrulline, glutamine and glutamate. Phenylalanine and glutamate were increased, while arginine, citrulline, ornithine and glutamine were decreased compared to levels after recovery. NO2/NO3 concentration in the urine, but not serum arginase activity, was significantly increased day 1 compared to day 5 after admission. Acute pancreatitis causes a disturbance of the serum amino acid spectrum, with possible implications for the inflammatory process and organ function both in the pancreas and the gut. Supplementation of selected amino acids could possibly be of value in this severe condition.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • WK Alderton CE Cooper RG Knowles (2001) ArticleTitleNitric oxide synthase: structure, function and inhibition Biochem J 357 593–615 Occurrence Handle11463332 Occurrence Handle10.1042/0264-6021:3570593 Occurrence Handle1:CAS:528:DC%2BD3MXmtFent70%3D

    Article  PubMed  CAS  Google Scholar 

  • K Ayub F Serracino-Inglott RCN Williamson RT Mathie (2001) ArticleTitleExpression of inducible nitric oxide synthase contributes to the development of pancreatitis following pancreatic ischaemia and reperfusion Br J Surg 88 1189–1193 Occurrence Handle11531865 Occurrence Handle10.1046/j.0007-1323.2001.01841.x Occurrence Handle1:STN:280:DC%2BD3MvovF2jsg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • V Bansal JB Ochoa (2003) ArticleTitleArginine availability, arginase and the immune response Curr Opin Clin Nutr Metab Care 6 223–228 Occurrence Handle12589193 Occurrence Handle10.1097/00075197-200303000-00012 Occurrence Handle1:CAS:528:DC%2BD3sXislejsr8%3D

    Article  PubMed  CAS  Google Scholar 

  • JL Boucher C Moali JP Tenu (1999) ArticleTitleNitric oxide biosynthesis, nitric oxide synthase inhibitors and arginase competition for L-arginine utilization Cell Mol Life Sci 55 1015–1028 Occurrence Handle10484661 Occurrence Handle10.1007/s000180050352 Occurrence Handle1:CAS:528:DyaK1MXks1Sltrw%3D

    Article  PubMed  CAS  Google Scholar 

  • Bradly EL 3rd (1993) A clinically based classification system for acute pancreatitis. Summary of the International Symposium on Acute Pancreatitis, Atlanta, GA, September 11 through 13. 1992 Arch Surg 128: 586–590

  • L Cynober J Le Boucher MP Vasson (1995) ArticleTitleArginine metabolism in mammals J Nutr Biochem 6 402 Occurrence Handle10.1016/0955-2863(95)00066-9 Occurrence Handle1:CAS:528:DyaK2MXntlSltbc%3D

    Article  CAS  Google Scholar 

  • DA De-Souza LJ Greene (2005) ArticleTitleIntestinal permeability and systemic infections in critically ill patients: effect of glutamine Crit Care Med 33 1125–1135 Occurrence Handle15891348 Occurrence Handle10.1097/01.CCM.0000162680.52397.97

    Article  PubMed  Google Scholar 

  • MB Gilliam MP Sherman JM Griscavage LJ Ignarro (1993) ArticleTitleA spectrophotometric assay for nitrate using NADPH oxidation by Aspergillus nitrate reductase Anal Biochem 212 359–363 Occurrence Handle8214577 Occurrence Handle10.1006/abio.1993.1341 Occurrence Handle1:CAS:528:DyaK3sXlsFGisbc%3D

    Article  PubMed  CAS  Google Scholar 

  • L Hagenfeldt A Arvidsson (1980) ArticleTitleThe distribution of amino acids in plasma and erytrocytes Clin Chim Acta 100 133–141 Occurrence Handle7351084 Occurrence Handle10.1016/0009-8981(80)90074-1 Occurrence Handle1:CAS:528:DyaL3cXhtVyhs7g%3D

    Article  PubMed  CAS  Google Scholar 

  • MM Hallemeesch WH Lamers NEP Deutz (2002) ArticleTitleReduced arginine availability and nitric oxide formation Clin Nutr 21 273–279 Occurrence Handle12135586 Occurrence Handle10.1054/clnu.2002.0571 Occurrence Handle1:CAS:528:DC%2BD38XmvVejtbs%3D

    Article  PubMed  CAS  Google Scholar 

  • JD Kruse-Jarres C Kaiser JC Hafkenscheid W Hohenwallner W Stein J Bohner G Klein W Poppe E Rauscher (1989) ArticleTitleEvaluation of a new alpha-amylase assay using 4.6-ethylidene-(G7)-1-4-nitrophenyl-(G1)-alpha-D-maltoheptaoside as substrate J Clin Chem Clin Biochem 27 103–113 Occurrence Handle2787387 Occurrence Handle1:CAS:528:DyaL1MXhvVyiu7o%3D

    PubMed  CAS  Google Scholar 

  • YC Luiking M Poeze CH Dejong G Ramsay NE Deutz (2004) ArticleTitleSepsis: an arginine deficiency state? Crit Care Med 32 2135–2145 Occurrence Handle15483426 Occurrence Handle10.1097/01.CCM.0000142939.81045.A0 Occurrence Handle1:CAS:528:DC%2BD2cXosVSltrk%3D

    Article  PubMed  CAS  Google Scholar 

  • A Mantovani S Sozzani M Locati P Allavena A Sica (2002) ArticleTitleMacrophage polarization: tumour-associated macrophages as a paradigm for polarized M2 mononuclear macrophages Trends Immunol 23 549–555 Occurrence Handle12401408 Occurrence Handle10.1016/S1471-4906(02)02302-5 Occurrence Handle1:CAS:528:DC%2BD38XotVKktrc%3D

    Article  PubMed  CAS  Google Scholar 

  • RS Mettu MD Wig M Khullar G Singh R Guptaa (2003) ArticleTitleEfficacy of serum nitric oxide level estimation in assessing the severity of necrotizing pancreatitis Pancreatology 3 506–514 Occurrence Handle14718751 Occurrence Handle10.1159/000076021 Occurrence Handle1:CAS:528:DC%2BD2cXht1Cnu7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • I Nissim (1999) ArticleTitleNewer aspects of glutamine/glutamate metabolism the role of acute pH changes Am J Physiol 277 493–497

    Google Scholar 

  • M Panteghini R Bonora (1996) ArticleTitleMeasurement of pancreatic lipase activity in serum by a kinetic colorimetric assay utilizing a chromogenic substrate reagent Clin Chem 42 101

    Google Scholar 

  • SH Rahman BJ Ammori M Larvin MJ McMahon (2003) ArticleTitleIncreased nitric oxide excretion in patients with severe acute pancreatitis: evidence of an endotoxin mediated inflammatory response? Gut 52 270–274 Occurrence Handle12524412 Occurrence Handle10.1136/gut.52.2.270 Occurrence Handle1:CAS:528:DC%2BD3sXhtlShs7k%3D

    Article  PubMed  CAS  Google Scholar 

  • WM Rand PL Pellett VR Young (2003) ArticleTitleMeta-analysis of nitrogen balance studies for estimating protein requirements in healthy adults Am J Clin Nutr 77 109–127 Occurrence Handle12499330 Occurrence Handle1:CAS:528:DC%2BD3sXhtFynsQ%3D%3D

    PubMed  CAS  Google Scholar 

  • M Rangel del Ara RA Gonzalez-Polo A Caro E Amo del L Paloma E Hernandez G Soler JM Fuentes (2002) ArticleTitleDiagnostic performance of arginase activity in colorectal cancer Clin Exp Med 1 53–57 Occurrence Handle10.1007/s102380200007

    Article  Google Scholar 

  • P Sandstrom T Gasslander T Sundqvist J Franke J Svanvik (2003) ArticleTitleDepletion of serum L-arginine in patients with acute pancreatitis Pancreas 9 261–266 Occurrence Handle10.1097/00006676-200310000-00012

    Article  Google Scholar 

  • P Sandstrom CM Woods M Brooke-Smith GTP Saccone J Toouli J Svanvik (2004) ArticleTitleHighly selective iNOS inhibition and sphincter of Oddi motility in the Australian possum ACTA Physiologica Scandinavia 181 321–331 Occurrence Handle10.1111/j.1365-201X.2004.01296.x Occurrence Handle1:CAS:528:DC%2BD2cXlslSqsbc%3D

    Article  CAS  Google Scholar 

  • PW Shaul (2002) ArticleTitleRegulation of endothelial nitric oxide synthase: location, location, location Annu Rev Physiol 64 749–774 Occurrence Handle11826287 Occurrence Handle10.1146/annurev.physiol.64.081501.155952 Occurrence Handle1:CAS:528:DC%2BD38XisFGmtr8%3D

    Article  PubMed  CAS  Google Scholar 

  • A Thune D Delbro B Nilsson S Friman J Svanvik (1995) ArticleTitleRole of nitric oxide in motility and seretion in the biliary tract Scand J Gastroenterol 30 715–720 Occurrence Handle7481537 Occurrence Handle10.3109/00365529509096318 Occurrence Handle1:CAS:528:DyaK2MXnt12nsro%3D

    Article  PubMed  CAS  Google Scholar 

  • P Vallance (2003) ArticleTitleNitric oxide: theraputic opportunities Fundam Clin Pharmacol 17 1–10 Occurrence Handle12588625 Occurrence Handle10.1046/j.1472-8206.2003.00124.x Occurrence Handle1:CAS:528:DC%2BD3sXhsFKlsLY%3D

    Article  PubMed  CAS  Google Scholar 

  • G Wu SM Morris (1998) ArticleTitleArginine metabolism: nitric oxide and beyond Biochem J 336 1–17 Occurrence Handle9806879 Occurrence Handle1:CAS:528:DyaK1cXotVGltrs%3D

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Authors’ address: Per Sandstrom, MD, PhD, Department of Biomedicine and Surgery, Faculty of Health Sciences, University Hospital, SE-581 85 Linköping, Sweden

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sandstrom, P., Trulsson, L., Gasslander, T. et al. Serum amino acid profile in patients with acute pancreatitis. Amino Acids 35, 225–231 (2008). https://doi.org/10.1007/s00726-007-0557-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00726-007-0557-5

Navigation