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Rapid, simple and effective technical procedure for the regeneration of IgG and HSA affinity columns for proteomic analysis

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Summary.

In plasma and serum, the presence of high-abundance proteins can overwhelm the signals of low-abundance proteins, which then become undetectable either by two-dimensional gels or chromatographic techniques. Therefore, depletion of abundant proteins is a prerequisite to detect low-abundance components. Furthermore, the regeneration of pre-purification tools could be money-saving. We applied an affinity chromatography kit to remove albumin and the immunoglobulin chains from plasma and propose a simple and effective technical procedure for the regeneration of these affinity columns.

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Authors and Affiliations

  1. Department of Clinical and Experimental Medicine, Chair of Metabolism, University of Padova, Padova, Italy

    R. Millioni, L. Puricelli, E. Iori & P. Tessari

Authors
  1. R. Millioni
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  2. L. Puricelli
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  3. E. Iori
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  4. P. Tessari
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Additional information

The first two authors have equally contributed to this work.

Authors’ address: Prof. Paolo Tessari, Dipartimento di Medicina Clinica e Sperimentale, Università di Padova, via Giustiniani 2, 35128 Padova, Italy

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Millioni, R., Puricelli, L., Iori, E. et al. Rapid, simple and effective technical procedure for the regeneration of IgG and HSA affinity columns for proteomic analysis. Amino Acids 34, 507–509 (2008). https://doi.org/10.1007/s00726-007-0556-6

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  • DOI: https://doi.org/10.1007/s00726-007-0556-6

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