Summary.
Our labs are focused on identifying amino acid sequences having the ability to react specifically with the functional binding site of a complementary antibody. Our epitopic definition is based on the analysis of the similarity level of antigenic amino acid sequences to the host proteome. Here, the similarity profile to the human proteome of an HCV E1 immunodominant epitope, i.e. the HCV E1315–328HRMAWDMMMNWSPT sequence, led to i) characterizing the immunoreactive HCV E1 315–328 region as a sequence endowed with a low level of similarity to human proteins; ii) defining 2 contiguous immunodominant linear determinants respectively located at the NH2 and COOH terminus of the conserved viral antigenic sequence. This study supports the hypothesis that low sequence similarity to the host’s proteome modulates the pool of epitopic amino acid sequences in a viral antigen, and appears of potential value in defining immunogenic viral peptide sequences to be used in immunotherapeutic approaches for HCV treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
T Allander K Drakenberg A Beyene D Rosa S Abrignani M Houghton A Widell L Grillner MA Persson (2000) ArticleTitleRecombinant human monoclonal antibodies against different conformational epitopes of the E2 envelope glycoprotein of hepatitis C virus that inhibit its interaction with CD81 J Gen Virol 81 2451–2459 Occurrence Handle10993933 Occurrence Handle1:CAS:528:DC%2BD3cXnt12hsro%3D
G Angelini D Bonamonte A Lucchese G Favia R Serpico A Mittelman S Simone AA Sinha D Kanduc (2006) ArticleTitlePreliminary data on Pemphigus vulgaris treatment by a proteomics-defined peptide: a case report J Transl Med 4 43 Occurrence Handle17062151 Occurrence Handle10.1186/1479-5876-4-43
F Bortolotti M Resti M Marcellini R Giacchino G Verucchi G Nebbia L Zancan MG Marazzi C Barbera A Maccabruni G Zuin G Maggiore F Balli P Vajro L Lepore M Molesini M Guido S Bartolacci F Noventa (2005) ArticleTitleHepatitis C virus (HCV) genotypes in 373 Italian children with HCV infection: changing distribution and correlation with clinical features and outcome Gut 54 852–857 Occurrence Handle15888796 Occurrence Handle10.1136/gut.2004.053744 Occurrence Handle1:STN:280:DC%2BD2M3kvFelsw%3D%3D
V Bottger EB Lane (1994) ArticleTitleA monoclonal antibody epitope on keratin 8 identified using a phage peptide library J Mol Biol 235 61–67 Occurrence Handle7507169 Occurrence Handle1:CAS:528:DyaK2cXitFCksrg%3D Occurrence Handle10.1016/S0022-2836(05)80013-0
X Forns J Bukh (1999) ArticleTitleThe molecular biology of hepatitis C virus. Genotypes and quasispecies Clin Liver Dis 3 693–716 Occurrence Handle11291246 Occurrence Handle10.1016/S1089-3261(05)70234-8 Occurrence Handle1:STN:280:DC%2BD3M3hslOnsw%3D%3D
C Gaboriaud V Bissery T Benchetrit JP Mornon (1987) ArticleTitleHydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences FEBS Lett 224 149–155 Occurrence Handle3678489 Occurrence Handle10.1016/0014-5793(87)80439-8 Occurrence Handle1:CAS:528:DyaL1cXjsVCntg%3D%3D
KG Hadlock RE Lanford S Perkins J Rowe Q Yang S Levy P Pileri S Abrignani SK Foung (2000) ArticleTitleHuman monoclonal antibodies that inhibit binding of hepatitis C virus E2 protein to CD81 and recognize conserved conformational epitopes J Virol 74 10407–10416 Occurrence Handle11044085 Occurrence Handle10.1128/JVI.74.22.10407-10416.2000 Occurrence Handle1:CAS:528:DC%2BD3cXnvF2rsb4%3D
A Lucchese J Willers A Mittelman D Kanduc R Dummer (2005) ArticleTitleProteomic scan for tyrosinase peptide antigenic pattern in vitiligo and melanoma. Role of sequence similarity and HLA-DR1 affinity J Immunol 175 7009–7020 Occurrence Handle16272362 Occurrence Handle1:CAS:528:DC%2BD2MXhtF2isr7M
Lucchese G, Stufano A, Trost B, Kusalik A, Kanduc D (2007) Peptidology: short amino acid modules in cell biology and immunology. Amino Acids (in press)
H Nagai K Matsumaru K Shiozawa K Momiyama N Wakui M Shinohara M Watanabe K Ishii H Nonaka A Hasegawa T Teramoto W Yamamuro Y Sumino K Miki (2005) ArticleTitleDisappearance of HCV after cessation of immunosuppression in a patient with ulcerative colitis and renal transplantation J Gastroenterol 40 848–853 Occurrence Handle16143892 Occurrence Handle10.1007/s00535-005-1640-x
C Natale T Giannini A Lucchese D Kanduc (2000) ArticleTitleComputer-assisted analysis of molecular mimicry between HPV16 E7 oncoprotein and human protein sequences Immunol Cell Biol 78 580–585 Occurrence Handle11114967 Occurrence Handle10.1046/j.1440-1711.2000.00949.x Occurrence Handle1:CAS:528:DC%2BD3cXovVegurY%3D
C Notredame DG Higgins J Heringa (2000) ArticleTitleT-Coffee: a novel method for multiple sequence alignments J Mol Biol 302 205–217 Occurrence Handle10964570 Occurrence Handle10.1006/jmbi.2000.4042 Occurrence Handle1:CAS:528:DC%2BD3cXmtVGntr8%3D
LV Olenina LI Nikolaeva BN Sobolev NP Blokhina AI Archakov EF Kolesanova (2002) ArticleTitleMapping and characterization of B cell linear epitopes in the conservative regions of hepatitis C virus envelope glycoproteins J Viral Hepat 9 174–182 Occurrence Handle12010504 Occurrence Handle10.1046/j.1365-2893.2002.00358.x Occurrence Handle1:STN:280:DC%2BD383nt1arsA%3D%3D
Y Tanaka F Kurbanov S Mano E Orito V Vargas JI Esteban MF Yuen CL Lai A Kramvis MC Kew HE Smuts SV Netesov HJ Alter M Mizokami (2006) ArticleTitleMolecular tracing of the global hepatitis C virus epidemic predicts regional patterns of hepatocellular carcinoma mortality Gastroenterology 130 703–714 Occurrence Handle16530512 Occurrence Handle10.1053/j.gastro.2006.01.032 Occurrence Handle1:CAS:528:DC%2BD28XjsVylsbs%3D
CH Wu H Huang L Arminski J Castro-Alvear Y Chen ZZ Hu RS Ledley KC Lewis HW Mewes BC Orcutt BE Suzek A Tsugita CR Vinayaka LS Yeh J Zhang WC Barker (2002) ArticleTitleThe protein information resource: an integrated public resource of functional annotation of proteins Nucleic Acids Res 30 35–37 Occurrence Handle11752247 Occurrence Handle10.1093/nar/30.1.35 Occurrence Handle1:CAS:528:DC%2BD38Xht12ksbk%3D
Author information
Authors and Affiliations
Additional information
Authors’ address: D. Kanduc, Department of Biochemistry and Molecular Biology, University of Bari, Via Orabona 4, Bari 70125, Italy
Rights and permissions
About this article
Cite this article
Polimeno, L., Mittelman, A., Gennero, L. et al. Sub-epitopic dissection of HCV E1315–328HRMAWDMMMNWSPT sequence by similarity analysis. Amino Acids 34, 479–484 (2008). https://doi.org/10.1007/s00726-007-0539-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00726-007-0539-7