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Protective effect of oxidative stress in HaCaT keratinocytes expressing E7 oncogene

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Summary.

In a previous study, we established a stable cell line which constitutively expresses E7 in HaCaT human keratinocyte cell line and identified various relevant factors including oxygen modulators affected by the E7 oncogene. E7-expressing HaCaT cells (HaCaT/E7) appeared to be more resistant to H2O2-induced cell death. Here, we demonstrate how E7 oncogene would modulate oxidative stress-induced cell death. In addition, we verified the increased expression of catalase in the HaCaT/E7 by Western blot analysis. The results suggest that the E7 oncogene would induce higher resistance to ROS-induced cell injury in the E7-infected cells via the upregulation of catalase. To investigate these paradoxical effects of high concentrations of H2O2 (500 µM–1 mM), we examined their effects on receptor mediated apoptosis, cell death via the mitochondrial pathway and modulation of apoptosis related factors. Our results revealed that HaCaT keratinocytes infected with HPV 16 E7 oncogene modulated expressions of catalase, Bcl-xL, IL-18, Fas, Bad, and cytochrome c as well as NF-κB, resulting in the resistance to oxidative stress-induced cell death.

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Abbreviations

EMSA:

electrophoretic mobility shift assay

HaCaT/E7:

HaCaT keratinocytes expressing E7 oncogene

HPV:

human papillomavirus

pRb:

retinoblastoma

ROS:

reactive oxygen species

RT-PCR:

reverse transcription-polymerase chain reaction

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Authors and Affiliations

  1. Laboratory of Cell and Immunobiochemistry, Division of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea

    J.-H. Shim, K.-H. Kim, H.-S. Choi & D.-Y. Yoon

  2. Laboratory of Molecular Pharmacology and Physiology, Medicinal Science Division, Korea Research Institute of Chemical Technology, Daejeon, Korea

    Y.-S. Cho

  3. Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea

    E. Y. Song

  4. Department of Pharmacy, College of Pharmacy, Chungnam National University, Daejeon, Korea

    P.-K. Myung

  5. Division of Genetic Toxicology, National Institute of Toxicological Research, Seoul, South Korea

    J. S. Kang, S.-K. Suh & S. N. Park

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  1. J.-H. Shim
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  2. K.-H. Kim
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  10. D.-Y. Yoon
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Corresponding author

Correspondence to D.-Y. Yoon.

Additional information

Authors’ address: Dr. Do-Young Yoon, Laboratory of Cell and Immunobiochemistry, Department of Bioscience and Biotechnology, Konkuk University, Hwayang-dong 1, Gwangjin-gu, Seoul 143-701, Korea

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Shim, JH., Kim, KH., Cho, YS. et al. Protective effect of oxidative stress in HaCaT keratinocytes expressing E7 oncogene. Amino Acids 34, 135–141 (2008). https://doi.org/10.1007/s00726-007-0499-y

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  • DOI: https://doi.org/10.1007/s00726-007-0499-y