Amino Acids

, Volume 32, Issue 4, pp 603–606 | Cite as

Protein-carbonyl accumulation in the non-replicative senescence of the methionine sulfoxide reductase A (msrA) knockout yeast strain

  • D. Oien
  • J. Moskovitz
Short Communication


The major enzyme of the methionine sulfoxide reductase (Msr) system is MsrA. Senescing msrA knockout mother yeast cells accumulated significant amounts of protein-carbonyl both at 5 generation-old (young) and 21 generation-old (old) cultures, while the control mother cells showed significant levels of protein-carbonyl mainly in the old culture. The Msr activities of both yeast strains declined with age and exposure of cells to H2O2 caused an accumulation of protein-carbonyl especially in the msrA knockout strain. It is suggested that a compromised MsrA activity may serve as a marker for non-replicative aging.

Keywords: Oxidative-stress – Non-replicative senescence – Post-translation modification 


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • D. Oien
    • 1
  • J. Moskovitz
    • 1
  1. 1.Department of Pharmacology and Toxicology, School of PharmacyUniversity of KansasLawrenceU.S.A.

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