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Detecting oxidative post-translational modifications in proteins

Summary.

Oxidative stress induces various post-translational modifications (PTM); some are reversible in vivo via enzymatic catalysis. The present paper reviews specific procedures for the detection of oxidative PTM in proteins, most of them including electrophoresis. Main topics are carbonylated and glutathionylated proteins as well as modification of selected amino acids (Cys, Tyr, Met, Trp, Lys).

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Gianazza, E., Crawford, J. & Miller, I. Detecting oxidative post-translational modifications in proteins. Amino Acids 33, 51–56 (2007). https://doi.org/10.1007/s00726-006-0410-2

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  • Keywords: Oxidative post-translational modifications – Proteomics – Electrophoresis – Carbonylation – Glutathionylation