Amino Acids

, 31:11 | Cite as

Nitric oxide and polyamine pathway-dependent modulation of neutrophil free amino- and α-keto acid profiles or host defense capability

  • J. Mühling
  • J. Engel
  • M. Halabi
  • M. Müller
  • M. Fuchs
  • M. Krüll
  • H. Harbach
  • T. W. Langefeld
  • M. Wolff
  • R. Matejec
  • I. D. Welters
  • T. Menges
  • G. Hempelmann


We have examined the effects of Nω-nitro-L-arginine-methylester-hydrochloride [L-NAME; inhibitor of nitric oxide synthase], S-nitroso-N-acetyl-penicillamine [SNAP; nitric oxide donor], α-difluoro-methyl-ornithine [DFMO; inhibitor of ornithine decarboxylase] arginine or ornithine as well as the combination of arginine or ornithine with L-NAME, SNAP or DFMO on intracellular free amino- and α-keto acid profiles and the immune function markers superoxide anion and hydrogen peroxide generation as well as released myeloperoxidase activity in neutrophils (PMN). Although the underlying mechanisms still remain unclear, we believe from our results that nitric oxide as well as polyamine-dependent pathways are involved in the signal transmission of free radical molecule, beneficial nutritional therapy or maleficient pharmacological stress-induced alterations in PMN nutrient composition. Relevant changes in intragranulocyte free amino- and α-keto acid homeostasis and metabolism, especially, may be one of the determinants in PMN nutrition that positively or negatively influences and modulate neutrophil host defence capability and immunocompetence.

Keywords: Nitric oxide – Polyamines – Neutrophil – Amino acids – α-Keto acids – Immune function 


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • J. Mühling
    • 1
  • J. Engel
    • 1
  • M. Halabi
    • 1
  • M. Müller
    • 1
  • M. Fuchs
    • 2
  • M. Krüll
    • 3
  • H. Harbach
    • 1
  • T. W. Langefeld
    • 1
  • M. Wolff
    • 1
  • R. Matejec
    • 1
  • I. D. Welters
    • 1
  • T. Menges
    • 1
  • G. Hempelmann
    • 1
  1. 1.Department of AnaesthesiologyIntensive Care Medicine, Pain Therapy, University Hospital Giessen and MarburgGiessenFederal Republic of Germany
  2. 2.Dr. Ing. Herbert Knauer GmbHBerlinFederal Republic of Germany
  3. 3.Department of Internal Medicine/Infectious Diseases, CharitéMedical School of Humboldt UniversityBerlinFederal Republic of Germany

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