Amino Acids

, Volume 29, Issue 3, pp 289–300 | Cite as

Alterations in neutrophil (PMN) free intracellular alpha-keto acid profiles and immune functions induced by L-alanyl-L-glutamine, arginine or taurine

  • J. Mühling
  • K. A. Nickolaus
  • M. Halabi
  • M. Fuchs
  • M. Krüll
  • J. Engel
  • M. Wolff
  • R. Matejec
  • T. W. Langefeld
  • I. D. Welters
  • T. Menges
  • M. G. Dehne
  • A. Sablotzki
  • G. Hempelmann


The objective of this study was to determine the dose as well as duration of exposure-dependent effects of L-alanyl-L-glutamine, arginine or taurine on polymorphonuclear neutrophil (PMN) free α-keto acid profiles and, in a parallel study, on PMN immune functions. Exogenous L-alanyl-L-glutamine significantly increased PMN α-ketoglutarate, pyruvate PMN superoxide anion (O2 ) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase (MPO) activity. Arginine also led to significant increases in α-ketoglutarate, pyruvate, MPO release and H2O2 generation. Formation of O2 on the other hand was decreased by arginine. Incubation with taurine resulted in lower intracellular pyruvate and α-ketobutyrate levels, decreased O2 and H2O2 formation and a concomitant significantly increased MPO activity. We therefore believe that considerable changes in PMN free-α-keto-acid profiles, induced for example by L-alanyl-L-glutamine, arginine or taurine, may be one of the determinants in cell nutrition that considerably modulates the immunological competence of PMN.

Keywords: L-Alanyl-L-glutamine – Arginine – Taurine – Neutrophil – α-Keto acids – Immune function – Superoxide anion – Hydrogen peroxide – Myeloperoxidase 


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Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • J. Mühling
    • 1
  • K. A. Nickolaus
    • 1
  • M. Halabi
    • 1
  • M. Fuchs
    • 2
  • M. Krüll
    • 3
  • J. Engel
    • 1
  • M. Wolff
    • 1
  • R. Matejec
    • 1
  • T. W. Langefeld
    • 1
  • I. D. Welters
    • 1
  • T. Menges
    • 1
  • M. G. Dehne
    • 1
  • A. Sablotzki
    • 4
  • G. Hempelmann
    • 1
  1. 1.Department of AnaesthesiologyIntensive Care Medicine, Pain Therapy, University Hospital, Justus Liebig UniversityGiessenGermany
  2. 2.Dr. Ing. Herbert Knauer GmbHBerlinGermany
  3. 3.Department of Internal Medicine/Infectious DiseasesCharité, Medical School of Humboldt UniversityBerlinGermany
  4. 4.Clinics of Anaesthesiology and Intensive Care Medicine, Martin Luther UniversityHalle-WittenbergGermany

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