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Amino Acids

, Volume 28, Issue 1, pp 85–98 | Cite as

Benzodiazepine receptor-dependent modulation of neutrophil (PMN) free amino- and α-keto acid profiles or immune functions

  • J. Mühling
  • J. Gonter
  • K. A. Nickolaus
  • R. Matejec
  • I. D. Welters
  • M. Wolff
  • A. Sablotzki
  • J. Engel
  • M. Krüll
  • T. Menges
  • M. Fuchs
  • M. G. Dehne
  • G. Hempelmann
Article

Summary.

We have examined the effects of midazolam, Ro 5-4864 (agonist for “peripheral” [p] benzodiazepine receptors [BR]), PK 11195 (antagonist for pBR), flumazenil (antagonist for “central” BR), naloxone (antagonist for opiate receptors) and the combination of midazolam and Ro 5-4864, PK 11195, flumazenil or naloxone on intracellular amino- and α-keto acids and the immune function markers superoxide anion (O2), hydrogen peroxide (H2O2) and released myeloperoxidase (MPO) activity in neutrophils (PMN). Only midazolam and Ro 5-4864 led to significant changes in the dynamic PMN free amino- and α-keto acid pools. Concerning PMN immune function markers, midazolam and Ro 5-4864 significantly decreased O2 and H2O2 formation and released MPO. When midazolam and Ro 5-4864 were applied together they appeared to act additively. Pre-incubation with PK 11195 partially neutralized the midazolam effects whereas flumazenil or naloxone showed no effects. We therefore believe that pBR are involved in the signal transmission of anesthetic-induced cellular metabolic changes in PMN.

Keywords: Benzodiazepine receptors – Neutrophil – Amino acids – α-Keto acids – Immune function 

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Copyright information

© Springer-Verlag/Wien 2004

Authors and Affiliations

  • J. Mühling
    • 1
  • J. Gonter
    • 1
  • K. A. Nickolaus
    • 1
  • R. Matejec
    • 1
  • I. D. Welters
    • 1
  • M. Wolff
    • 1
  • A. Sablotzki
    • 3
  • J. Engel
    • 1
  • M. Krüll
    • 4
  • T. Menges
    • 1
  • M. Fuchs
    • 2
  • M. G. Dehne
    • 1
  • G. Hempelmann
    • 1
  1. 1.Department of Anaesthesiology, Intensive Care Medicine, Pain TherapyUniversity Hospital, Justus Liebig UniversityGiessenGermany
  2. 2.Dr. Ing. Herbert Knauer GmbHBerlinGermany
  3. 3.Clinics of Anaesthesiology and Intensive Care Medicine, Martin Luther UniversityHalle-WittenbergGermany
  4. 4.Department of Internal Medicine/Infectious Diseases, CharitéMedical School of Humboldt UniversityBerlinGermany

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