Advertisement

Amino Acids

, Volume 27, Issue 3–4, pp 313–319 | Cite as

Effects of ornithine on neutrophil (PMN) free amino acid and α-keto acid profiles and immune functions in vitro

  • J. Mühling
  • M. Fuchs
  • M. Campos
  • J. Gonter
  • A. Sablotzki
  • J. Engel
  • I. D. Welters
  • M. Wolff
  • R. Matejec
  • M. G. Dehne
  • T. Menges
  • M. Krüll
  • G. Hempelmann
Article

Summary.

The objective of this study was to determine the effects of ornithine on polymorphonuclear leucocyte (PMN) free amino- and α-keto acid profiles, superoxide anion (O2) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase acitivity (MPO). Exogenous ornithine significantly increased PMN asparagine, glutamine, asparatate, glutamate, arginine, citrulline, alanine, α-ketoglutarate and pyruvate as intracellular ornithine increased. Concerning PMN immune function markers ornithine increased H2O2-generation and MPO acitivity while O2-formation was decreased. We believe therefore that ornithine is important for affecting PMN “susceptible free amino- and α-keto acid pool” although the mechanisms are not yet clear. This may be one of the determinants in PMN nutrition considerably influencing and modulating PMN host defense capability.

Keywords: Ornithine – Neutrophil – Amino acids – α-Keto acids – Immune function 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag/Wien 2004

Authors and Affiliations

  • J. Mühling
    • 1
  • M. Fuchs
    • 2
  • M. Campos
    • 2
  • J. Gonter
    • 1
  • A. Sablotzki
    • 3
  • J. Engel
    • 1
  • I. D. Welters
    • 1
  • M. Wolff
    • 1
  • R. Matejec
    • 1
  • M. G. Dehne
    • 1
  • T. Menges
    • 1
  • M. Krüll
    • 4
  • G. Hempelmann
    • 1
  1. 1.Department of Anaesthesiology, Intensive Care Medicine, Pain TherapyUniversity Hospital, Justus Liebig UniversityGiessenGermany
  2. 2.Dr. Ing. Herbert Knauer GmbHBerlinGermany
  3. 3.Clinics of Anaesthesiology and Intensive Care Medicine, Martin Luther UniversityHalle-WittenbergGermany
  4. 4.Department of Internal Medicine/Infectious DiseasesCharité, Medical School of Humboldt UniversityBerlinGermany

Personalised recommendations