Skip to main content
SpringerLink
Log in

A Study to Identify and Analyze the Radiosensitivity of 4-Piperidinecarboxylic Acid Using Electron Paramagnetic Resonance Spectroscopy

  • Original Paper
  • Published:
Applied Magnetic Resonance Aims and scope Submit manuscript

Abstract

This study aims to investigate the spectroscopic, dosimetric, and stability properties of free radicals generated within 4-piperidinecarboxylic acid (4PCA) sample due to gamma irradiation within the 2–20 kGy range, utilizing electron paramagnetic resonance (EPR) spectroscopy. To achieve this goal, the EPR signals of the samples were studied in relation to microwave power, ranging from 0.01 to 50 mW, at room temperature. The investigation revealed that γ-irradiation induces the formation of –CH2ĊCH2CH2– (radical I) and –ĊHN– (radical II) radicals within the 4PCA compound. Hyperfine structure constants and g values were also determined for the radicals. The optimal agreement between experimental and computed results within the dose range of 2–20 kGy was achieved using polynomial and linear functions. Experimental measurements were undertaken under varied temperature conditions (123–323 K) to study the dosimetric features of free radicals generated as a consequence of gamma irradiation. Furthermore, an investigation into the time dependency of signal intensity within the irradiated sample was conducted, with the objective of analyzing the long-term stability characteristics of the free radicals present.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Availability of Data and Materials

The author confirms that the datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. F.A. Taiwo, L.H. Patterson, E. Jaroszkiewicz, B. Marciniec, M. Ogrodowczyk, Free radicals in irradiated drugs: an EPR study. Free Radic. Res. 31, 231–235 (1999)

    Article  Google Scholar 

  2. J.P. Basly, M. Bernard, I. Basly, Electron spin resonance detection of radiosterilization of pharmaceuticals: application to four nitrofurans. Analyst 123, 1753–1756 (1998)

    Article  ADS  Google Scholar 

  3. L. Varshney, P.B. Dodke, Radiation effect studies on anticancer drugs, cyclophosphamide and doxorubicin for radiation sterilization. Radiat. Phys. Chem. 71, 1103–1111 (2004)

    Article  ADS  Google Scholar 

  4. J.P. Basly, I. Longy, M. Bernard, Radiosterilization dosimetry by electron-spin resonance spectroscopy: cefotetan. Anal. Chim. Acta 359, 107–113 (1998)

    Article  Google Scholar 

  5. J.P. Basly, I. Basly, M. Bernard, Radiation induced effects on cephalosporins: an ESR study. Int. J. Radiat. Biol. 75, 259–263 (1999)

    Article  Google Scholar 

  6. B.R. Aşik, E. Aras, M. Birey, M. Eken, EPR study of γ-irradiated N-hydroxysuccinimide. Radiat. Eff. Defects Solids 163, 71–77 (2008)

    Article  ADS  Google Scholar 

  7. J.P. Basly, I. Longy, M. Bernard, Radiation effects on dopamine and norepinephrine. Pharm. Res. 14, 1192–1196 (1997)

    Article  Google Scholar 

  8. H.S. Murrieta, E.P. Munoz, E. Adem, G. Burillo, M. Vazquez, E.B. Cabrera, Effect of irradiation dose, storage time and temperature on the ESR signal in irradiated oat, and corn and wheat. App. Radiat. Isotopes. 47, 1657–1661 (1996)

    Article  Google Scholar 

  9. S. Onori, M. Pantaloni, P. Fattibene, E.C. Signoretti, L. Valvo, M. Santucci, ESR identification of irradiated antibiotics: cephalosporins. App. Radiat. Isotopes. 47, 1569–1572 (1996)

    Article  Google Scholar 

  10. H.B. Ambroż, E.M. Kornacka, B. Marciniec, M. Ogrodowczyk, G.K. Przybytniak, EPR study of free radicals in some drugs γ-irradiated in the solid state. Radiat. Phys. Chem. 58, 357–366 (2000)

    Article  ADS  Google Scholar 

  11. E. Emel, O. Ayhan, B. Recep, S. Ulku, Gamma irradiation effect on some asthma drugs: EPR detection of radiosterilization. Radiat. Protect. Dosimetry. 199, 1600–1604 (2023)

    Article  Google Scholar 

  12. K.I. Aleksieva, N.D. Yordanov, Various approaches in EPR identification of gamma-irradiated plant foodstuffs: a review. Food Res. Int. 105, 1019–1028 (2018)

    Article  Google Scholar 

  13. K. Aleksieva, L. Georgieva, E. Tzvetkova, N.D. Yordanov, EPR study on tomatoes before and after gamma-irradiation. Radiat. Phys. Chem. 78, 823–825 (2009)

    Article  ADS  Google Scholar 

  14. M.H. Başkan, Y.E. Osmanoğlu, K. Sütçü, M. Aydın, Ş Osmanoğlu, Radiation effect studies in single crystal of Trifluoroacetyl-α-Aminoisobutyric acid. Radiat. Effect. Defect. Solids. 170, 854–861 (2015)

    Article  ADS  Google Scholar 

  15. Ş Osmanoğlu, M. Aydın, Y.E. Osmanoğlu, I.Y. Dicle, M.H. Başkan, Structure and behaviour of the free radicals generated in gamma irradiated amino acid and iminodiacetic acid derivatives. Acta Part A Mol. Biomol. Spectro. 78, 1611–1614 (2011)

    Article  ADS  Google Scholar 

  16. H. Ambroż, E. Kornacka, B. Marciniec, G. Przybytniak, Radical decay in irradiated drugs: flutamide, ifosfamide. J. Radioanal. Nucl. Chem. 254, 293–298 (2002)

    Article  Google Scholar 

  17. K. Sütçü, Y.E. Osmanoğlu, Identification of free radicals formed in gamma-irradiated acetazolamide using electron paramagnetic resonance spectroscopy. App. Mag. Res. 54, 1–14 (2023)

    Google Scholar 

  18. G. Damian, EPR investigation of γ-irradiated anti-emetic drugs. Talanta 60, 923–927 (2003)

    Article  Google Scholar 

  19. L. Jiménez-Hernández, O. Estévez-Hernández, M.P. Hernández, J.A. Díaz, M.F. Farías, E. Reguera, On the state of Mn in MnxZn1 − xO nanoparticles and their surface modification with isonipecotic acid. J. Solid State Chem. 247, 43–52 (2017)

    Article  ADS  Google Scholar 

  20. M. Mortensen, U. Kristiansen, B. Ebert, B. Frølund, P. Krogsgaard-Larsen, T.G. Smart, Activation of single heteromeric GABAA receptor ion channels by full and partial agonists. J. Physiol. 557, 393–417 (2004)

    Article  Google Scholar 

  21. A.M. Dam, L.G. Gazso, S. Kaewpila, I. Maschek, Radiation sterilization dose calculation for heparin and aprotinin based on ISO Method 1. Int. J. Pharm. 121(2), 245–248 (1995)

    Article  Google Scholar 

  22. NIH 2012. Public electron paramagnetic resonance software tools, https://www.niehs.nih.gov/research/resources/ software/tox-pharm/tools/index.Cfm.

  23. K. Sütçü, Y.E. Osmanoğlu, Electron spin resonance identification of gamma irradiated 2, 4′-dichlorodiphenyl dichloroethane and 6-mercaptopurine monohydrate in the solid state. J. Mol. Struc. 1174, 67–73 (2018)

    Article  ADS  Google Scholar 

  24. S. Wilczyński, B. Pilawa, R. Koprowski, Z. Wróbel, M. Ptaszkiewicz, J. Swakoń, J.P. Olko, EPR studies of free radicals decay and survival in gamma irradiated aminoglycoside antibiotics: sisomicin, tobramycin and paromomycin. Eur. J. Pharm. Sci. 45, 251–262 (2012)

    Article  Google Scholar 

  25. R. Köseoǧlu, E. Köseoǧlu, F. Köksal, Electron paramagnetic resonance of some γ-irradiated drugs. App. Radiat. Isotop. 58, 63–68 (2003)

    Article  Google Scholar 

  26. S. Iravani, G.J. Soufi, Electron paramagnetic resonance (EPR) spectroscopy: food, biomedical and pharmaceutical analysis. Biomed. Spec. Imaging 9, 165–182 (2020)

    Article  Google Scholar 

  27. C. Aydaş, M. Polat, M. Korkmaz, Identification and dosimetric features of γ-irradiated cefadroxil by electron spin resonance. Radiat. Phys. Chem. 77, 79–86 (2008)

    Article  ADS  Google Scholar 

Download references

Funding

This work was supported by Grant no. ZGEF.22.003 of research Fund of Dicle University (DUBAP).

Author information

Authors and Affiliations

  1. Department of Science, Faculty of Education, Dicle University, 21280, Diyarbakır, Turkey

    Kerem Sütçü

Authors
  1. Kerem Sütçü
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

I, KS, am solely author of this manuscript.

Corresponding author

Correspondence to Kerem Sütçü.

Ethics declarations

Conflict of interest

The author has no relevant financial or non-financial interests to disclose.

Ethical approval

This research did not contain any studies involving animal or human participants, nor did it take place on any private or protected areas. No specific permissions were required for corresponding locations.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sütçü, K. A Study to Identify and Analyze the Radiosensitivity of 4-Piperidinecarboxylic Acid Using Electron Paramagnetic Resonance Spectroscopy. Appl Magn Reson (2024). https://doi.org/10.1007/s00723-024-01641-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00723-024-01641-3

Search

Navigation