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Impurities of [1-13C]Pyruvic Acid and a Method to Minimize Their Signals for Hyperpolarized Pyruvate Metabolism Studies

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Abstract

Impurities are a necessary evil when working with hyperpolarized [1-13C]pyruvate for real-time metabolic studies. We show that the impurities’ spectrum persists throughout the detection window as these signals show a long T1. At the same time, the impurities’ spectrum is unpredictable and partly susceptible to minute pH changes in the microenvironment such that impurities’ signals may overlap with hyperpolarized metabolite signals. We show a method to eliminate the hyperpolarized impurities’ signals while maintaining sufficient hyperpolarized [1-13C]pyruvate signal for metabolic studies.

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Funding

Sponsor: This project has received funding from the European Research Council (ERC) under grant agreement No. 338040 to R.K-B) and from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 667192.

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Authors and Affiliations

  1. Department of Radiology, Hadassah-Hebrew University Medical Center, POB 12000, 9112001, Jerusalem, Israel

    Talia Harris, Ayelet Gamliel, Jacob Sosna, J. Moshe Gomori & Rachel Katz-Brull

Authors
  1. Talia Harris
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  2. Ayelet Gamliel
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  3. Jacob Sosna
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  4. J. Moshe Gomori
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  5. Rachel Katz-Brull
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Correspondence to Rachel Katz-Brull.

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Harris, T., Gamliel, A., Sosna, J. et al. Impurities of [1-13C]Pyruvic Acid and a Method to Minimize Their Signals for Hyperpolarized Pyruvate Metabolism Studies. Appl Magn Reson 49, 1085–1098 (2018). https://doi.org/10.1007/s00723-018-1030-1

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  • DOI: https://doi.org/10.1007/s00723-018-1030-1

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