Skip to main content
SpringerLink
Log in

Proton NMR relaxation in albumin solutions doped with Mn(II)

  • Published:
Applied Magnetic Resonance Aims and scope Submit manuscript

Abstract

The aim of this study is to obtain further information about the source of proton relaxation in the Mn(II)-human serum albumin complex. For this purpose, proton relaxation rates in albumin solutions 1/T 1 and 1/T 2 were measured versus increasing amounts of manganese [Mnt]. The fractions of manganese bound to albumin [Mnb] and free manganese [Mnf] were then determined from proton relaxation rate enhancement data. Paramagnetic contributions of bound manganese to the observed relaxation rates (1/T *1p )b and (1/T *2p )b were also determined. Finally, the (1/T *2p )b/(1/T *1p )b ratio was used in a derived equation to estimate an effective correlation time τ. Mean τ value of the complex was found to be in the order of 3 ns, while the hydration number of bound manganese q was estimated to be about 4. The 1/τ was found to be the sum of the inverse values of rotational correlation time 1/τ r, mean residence time of water in hydration spheres of the complex 1/τ m, and longitudinal electronic relaxation time of manganese 1/τ s in the complex. In conclusion, the relaxation mechanism in albumin solutions containing Mn(II) can be interpreted through dipolar and scalar interactions modulated by τ r, τ m and τ s. This analysis enables one to get reasonable figures for the τ r and q of Mn(II) in albumin solution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cistola, D.P., Small, D.M.: J. Clin. Invest. 87, 1431–1441 (1991)

    Article  Google Scholar 

  2. Fasano, M., Curry, S., Terreno, E., Galliano, M., Fanali, G., Narciso, P., Notari, S., Ascenzi, P.: IUBMB Life 12, 787–796 (2005)

    Article  Google Scholar 

  3. Aime, S., Canton, S., Crich, S.G., Terreno, E.: Magn. Reson. Chem. 40, 41–48 (2002)

    Article  Google Scholar 

  4. Aime, S., Botta, M., Fasano, M., Geninatti, C., Terreno, E.: J. Biol. Inorg. Chem. 1, 312–319 (1996)

    Article  Google Scholar 

  5. Yushmanov, V.E., Tominaga, T.T., Borissevitch, I.E., Imasato, H., Tabak, M.: Magn. Reson. Imaging 14, 255–261 (1996)

    Article  Google Scholar 

  6. Caravan, P., Cloutier, N.J., Greenfield, M.T., McDermid, S.A., Dunham, S.U., Bulte, J.W.M., Lauffer, R.B.: J. Am. Chem. Soc. 124, 3152–3162 (2002)

    Article  Google Scholar 

  7. Parac-Vogt, T.N., Kimpe, K., Laurent, S.: Chemistry 10, 3077–3086 (2005)

    Google Scholar 

  8. Nandedkar, A.K.N., Nurse, C.E., Friedberg, F.: Int. J. Pept. Protein Res. 5, 279–281 (1973)

    Google Scholar 

  9. Mildvan, A.S., Cohn, M.: Biochemistry 2, 910–919 (1963)

    Article  Google Scholar 

  10. Sherry, A.D., Birnbaum, E.R., Darnall, D.W.: Anal. Biochem. 52, 415–420 (1973)

    Article  Google Scholar 

  11. Budak, H.: Z Naturforsch. C 60, 807–812 (2005)

    Google Scholar 

  12. Van-Quynh, A., Willson, S., Bryant, R.G.: Biophys. J. 84, 558–563 (2003)

    Article  ADS  Google Scholar 

  13. Jones, R., Dwek, R.A., Forsen, S.: Eur. J. Biochem. 47, 271–283 (1974)

    Article  Google Scholar 

  14. Peacocke, A.R., Richards, R.E., Sheard, B.: Mol. Phys. 16, 177–188 (1969)

    ADS  Google Scholar 

  15. Navon, G.: Chem. Phys. Lett. 7, 390–394 (1970)

    Article  ADS  Google Scholar 

  16. Reuben, J., Cohn, M.: J. Biol. Chem. 245, 6539–6546 (1970)

    Google Scholar 

  17. Lanir, A., Gradstajn, S., Navon, G.: Biochemistry 14, 242–248 (1975)

    Article  Google Scholar 

  18. Fushman, D., Weisemann, R., Thuring, H., Ruterjans, H.: J. Biomol. NMR 4, 61–78 (1994)

    Article  Google Scholar 

  19. Yılmaz, A., Köylü, M.Z., Budak, H.: Chem. Phys. Lett. 427, 346–349 (2006)

    Article  ADS  Google Scholar 

  20. Kingsley, P.B.: Concepts Magn. Res. 11, 243–276 (1999)

    Article  Google Scholar 

  21. Fleysher, L., Fleysher, R., Lui, S., Zaaraoui, W., Gonen, O.: Magn. Reson. Med. 57, 380–387 (2007)

    Article  Google Scholar 

  22. Kang, Y.S., Gore, J.C., Armitage, I.M.: Magn. Reson. Med. 1, 396–409 (1984)

    Article  Google Scholar 

  23. Barnhart, J.L., Berk, R.N., Andre, M.: Physiol. Chem. Phys. Med. NMR 17, 53–60 (1985)

    Google Scholar 

  24. Barnhart, J.L., Berk, R.N.: Invest. Radiol. 21, 132–136 (1986)

    Article  Google Scholar 

  25. Ylmaz, A., Yurdakoç, M., Işık, B.: Biol. Trace Elem. Res. 67, 187–193 (1999)

    Article  Google Scholar 

  26. Suzanne, K., Bryant, R.G.: Biophys. J. 78, 2163–2169 (2000)

    Article  Google Scholar 

  27. Jones, R., Dwek, R.A., Walker, O.: Eur. J. Biochem. 47, 285–293 (1974)

    Article  Google Scholar 

  28. Slone, R.M., Fitzsimmons, J.R.: Med. Phys. 14, 131–134 (1987)

    Article  Google Scholar 

  29. Halle, B.: Philos. Trans. R. Soc. B 359, 1207–1224 (2004)

    Article  Google Scholar 

  30. Vander Elst, L., Laurent, S., Masaka Bintoma, H., Muller, R.N.: MAGMA 12, 135–140 (2001)

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physics, Faculty of Science, University of Dicle, 21280, Diyarbakir, Turkey

    Ali Yilmaz & M. Z. Koylu

  2. Division of Statistics, Professional High School, University of Dicle, Diyarbakir, Turkey

    E. Uysal

Authors
  1. Ali Yilmaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Z. Koylu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Uysal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ali Yilmaz.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yilmaz, A., Koylu, M.Z. & Uysal, E. Proton NMR relaxation in albumin solutions doped with Mn(II). Appl Magn Reson 35, 601–612 (2009). https://doi.org/10.1007/s00723-009-0189-x

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00723-009-0189-x

Keywords

Search

Navigation