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EPR Spectra of Spin-labeled Fatty Acids in Detergent Solutions: “Immobilized Species” in the Absence of Protein

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Abstract

Electron paramagnetic resonance spectra of 5-doxyl-stearic acid, 3-(2-Dodecyl-1-oxyl-5,5-dimethylpyrrolidin-2-yl)propanoic acid radical (4-proxyl-palmitic acid) and of 3-(1-Oxyl-2,5-dimethyl-5-dodecylpyrrolidine-2-yl)propanoic acid radical (4-azetoxyl-stearic acid) in lipid micelles of nonaethyleneglycol-monododecyl ether (C12E9) and pentaethyleneglycol-monooctyl ether (C8E5) can reveal immobilized components typical of spin-labeled protein-bound fatty acids as well as phospholipids. The occurrence of such species depends on the detergent, the relative orientation of the nitroxide group with respect to the longitudinal axis and the molecular structure of the fatty acid as well as on polarity and ionic strength of the aqueous phase. Whereas 5-doxyl-stearic acid and 4-proxyl-palmitic acid exhibited these highly immobilized components in C12E9 micelles but not in C8E5, only a single relatively mobile component was observed with 4-azetoxyl-stearic acid in both detergents. The immobilized component is lost by the addition of isopropanol but not as much by ethanol and also when distilled water is substituted for the buffer.

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Authors and Affiliations

  1. Department of Chemistry, Kaiserslautern University of Technology, Postfach 3049, 67653, Kaiserslautern, Germany

    Stefan Kuntz, Fabian Leinisch & Wolfgang E. Trommer

  2. Institute of Organic and Medicinal Chemistry, University of Pécs, Pecs, Hungary

    Kálmán Hideg

Authors
  1. Stefan Kuntz
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  2. Fabian Leinisch
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  3. Kálmán Hideg
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  4. Wolfgang E. Trommer
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Correspondence to Wolfgang E. Trommer.

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Kuntz, S., Leinisch, F., Hideg, K. et al. EPR Spectra of Spin-labeled Fatty Acids in Detergent Solutions: “Immobilized Species” in the Absence of Protein. Appl Magn Reson 37, 455–461 (2010). https://doi.org/10.1007/s00723-009-0057-8

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  • DOI: https://doi.org/10.1007/s00723-009-0057-8

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