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p-Nitrostilbene-tert-Butyl-Nitrone: a Novel Fluorescent Spin Trap for the Detection of ROS with Subcellular Resolution

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Abstract

A fluorescent nitrone composed of a nitrostilbene moiety and the tert-butyl-nitrone has been synthesized. Upon addition of short-lived oxygen radicals a relatively stable nitroxide is formed which quenches the fluorescence. Simultaneously, the fluorescence maximum is shifted to shorter wavelength due to the shorter conjugated system. Hence, by means of confocal laser microscopy the formation of reactive oxygen species can be followed with subcellular resolution. The probe co-localizes with mitochondria. Quench was followed in Chinese hamster ovary cells on the second time scale either after generation of hydroxyl radicals by the Fenton reaction or, at almost the same rate, by blocking complexes I and III of the respiratory chain by rotenone and antimycin A. The fluorescence lasted for more than 20 min in controls. The fluorescence decay can be followed in a video presentation on our homepage (http://pcbc00.chemie.uni-kl.de/videostream/stream2java.html). The nature of the initial radical may eventually be determined by electron paramagnetic resonance spectroscopy of the adduct.

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Acknowledgments

We want to thank Prof. Gertz Likhtenshtein, Ben Gurion University of the Negev, Israel, as well as Dr. Vlad Papper and Dr. Matthias Schneider for their initial suggestion to use stilbenes as fluorophores and the synthesis of an unsubstituted stilbene nitrone in our laboratory.

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Authors and Affiliations

  1. Department of Chemistry, Technical University Kaiserslautern, P.O.Box 3049, 67653, Kaiserslautern, Germany

    Stefan Hauck, Yvonne Lorat, Fabian Leinisch & Wolfgang E. Trommer

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  1. Stefan Hauck
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  2. Yvonne Lorat
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  3. Fabian Leinisch
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  4. Wolfgang E. Trommer
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Correspondence to Wolfgang E. Trommer.

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Hauck, S., Lorat, Y., Leinisch, F. et al. p-Nitrostilbene-tert-Butyl-Nitrone: a Novel Fluorescent Spin Trap for the Detection of ROS with Subcellular Resolution. Appl Magn Reson 36, 133–147 (2009). https://doi.org/10.1007/s00723-009-0025-3

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