Abstract.
900 MHz 1H NMR (nuclear magnetic resonance) spectroscopy was used to analyze metabolites in allantoic fluid of the chicken embryo in the 9th incubation day without any chemical or physical sample separation. The result showed that superhigh spectral dispersion and sensitivity at 900 MHz are useful in directly analyzing a complex mixture, particularly in resolving the overlapped NMR resonances. In this study, we had assigned 61 metabolites and 26 chemical fragments in the allantoic fluid. The relative concentrations of the major metabolites were also measured. Among the assigned metabolites, some were detected by NMR in chicken embryo allantoic fluids for the first time. The biological functions of these compounds were briefly discussed.
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Authors' address: Maili Liu, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China
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Feng, Y., Zhang, X., Ye, C. et al. Analysis of Metabolites in Allantoic Fluid of Chicken Embryo by 900 MHz NMR Spectroscopy. Appl Magn Reson 32, 257–268 (2007). https://doi.org/10.1007/s00723-007-0027-y
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DOI: https://doi.org/10.1007/s00723-007-0027-y