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Enhanced ultraviolet-B radiation alleviates structural damages on rice leaf caused by Magnaporthe oryzae infection

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Abstract

Enhanced ultraviolet-B (UV-B) radiation can change the interaction between crops and pathogens. The effects of single and compound stresses of enhanced UV-B radiation (5.0 kJ·m−2) and Magnaporthe oryzae on the morphology, anatomy, and ultrastructure of rice leaves were investigated. M. oryzae infection decreased the leaf area and thickness, reduced the stomatal area and density, and caused damages to the leaf ultrastructure, such as cytoplasm–cell wall separation, atrophy and sinking of fan-shaped bulliform cells, and chloroplast deformation. The enhanced UV-B radiation supplied before or during M. oryzae infection remarkably decreased the mycelia number of M. oryzae in leaf epidermis, increased the leaf area, leaf thickness, stomatal density, and mastoid number; and alleviated the ultrastructural damages induced by M. oryzae to keep an integral chloroplast. While the UV-B radiation was supplied after M. oryzae infection, its alleviation effects on the damages induced by M. oryzae infection on the morphology and structure of rice leaf were attenuated. Thus, the alleviation of enhanced UV-B radiation on damages induced by M. oryzae infection on rice leaves was related to its application period. The enhanced UV-B radiation supplied before or during M. oryzae infection allowed the rice leaf to resist M. oryzae infection.

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Funding

This work was financially supported by the National Natural Science Foundation of China (Nos. 32060287 and 31760113) and the Reserve Talents Fund for Young and Middle-Aged Academic and Technological Leaders in Yunnan Province (202005AC160038).

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  1. Yongmei He and Hongru Li have contributed equally to this work.

Authors and Affiliations

  1. College of Resources and Environment, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Yongmei He, Hongru Li, Jiong Wu, Xiang Li, Yanqun Zu, Fangdong Zhan & Yuan Li

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  1. Yongmei He
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  2. Hongru Li
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  3. Jiong Wu
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  4. Xiang Li
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  5. Yanqun Zu
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  6. Fangdong Zhan
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  7. Yuan Li
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Contributions

Yongmei He and Hongru Li contributed on experiments, data analysis, and manuscript writing. Jiong Wu and Xiang Li contributed on experiment methodology. Yanqun Zu and Fangdong Zhan contributed on experimental design. Yuan Li contributed on funding acquisition and writing revision.

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Correspondence to Yuan Li.

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He, Y., Li, H., Wu, J. et al. Enhanced ultraviolet-B radiation alleviates structural damages on rice leaf caused by Magnaporthe oryzae infection. Protoplasma 261, 161–171 (2024). https://doi.org/10.1007/s00709-023-01867-1

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