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The opening of mitochondrial permeability transition pore (mPTP) and the inhibition of electron transfer chain (ETC) induce mitophagy in wheat roots under waterlogging stress

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Abstract

Mitochondria are crucial for the regulation of intracellular energy metabolism, biosynthesis, and cell survival. And studies have demonstrated the role of mitochondria in oxidative stress–induced autophagy in plants. Previous studies found that waterlogging stress can induce the opening of mitochondrial permeability transition pore (mPTP) and the release of cytochrome c in endosperm cells, which proved that mPTP plays an important role in the programmed cell death of endosperm cells under waterlogging stress. This study investigated the effects of the opening of mPTP and the inhibition of ETC on mitophagy in wheat roots under waterlogging stress. The results showed that autophagy related genes in the mitochondria of wheat root cells could respond to waterlogging stress; waterlogging stress led to the degradation of the characteristic proteins cytochrome c and COXII in the mitochondria of root cells. With the prolongation of waterlogging time, the protein degradation degree and the occurrence of mitophagy gradually increased. Under waterlogging stress, exogenous mPTP opening inhibitor CsA inhibited mitophagy in root cells and alleviated mitophagy induced by flooding stress, while exogenous mPTP opening inducer CCCP induced mitophagy in root cells; exogenous mPTP opening inducer CCCP induced mitophagy in root cells. The electron transfer chain inhibitor antimycin A induces mitophagy in wheat root cells and exacerbates mitochondrial degradation. In conclusion, waterlogging stress led to the degradation of mitochondrial characteristic proteins and the occurrence of mitophagy in wheat root cells, and the opening of mPTP and the inhibition of ETC induced the occurrence of mitophagy.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Abbreviations

mPTP:

Mitochondrial permeability transition pore

ETC:

Electron transfer chain

ATG:

Autophagy-related gene

MDC:

Dansylcadaverine

CsA:

Ciclosporin A

CCCP:

Carbonyl cyanide 3-chlorophenylhydrazone

AA:

Antimycin A

PCD:

Programmed cell death

PBS:

Phosphate buffered saline

PAGE:

Polyacrylamide gel electrophoresis

ROS:

Reactive oxygen species

TEM:

Transmission electron microscope

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Funding

This work was supported by the National Natural Foundation of China (Grant No.31871530).

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Authors and Affiliations

  1. College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Yueli Wang, Hailong Ye, Kaiyue Gao, Gege Li, Qiutao Xu & Zhuqing Zhou

  2. College of Food and Biological Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Xiangyi Deng

  3. College of Food and Biological Science and Technology, Wuhan Institute of Design and Sciences, Wuhan, 430070, Hubei, China

    Jiwei Li

  4. Division of Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Fangzhu Mei

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  1. Yueli Wang
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Wang, Y., Ye, H., Gao, K. et al. The opening of mitochondrial permeability transition pore (mPTP) and the inhibition of electron transfer chain (ETC) induce mitophagy in wheat roots under waterlogging stress. Protoplasma 260, 1179–1191 (2023). https://doi.org/10.1007/s00709-022-01834-2

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