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At3g53630 encodes a GUN1-interacting protein under norflurazon treatment

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Abstract

Chloroplasts are semi-autonomous organelles, with more than 95% of their proteins encoded by the nuclear genome. The chloroplast-to-nucleus retrograde signals are critical for the nucleus to coordinate its gene expression for optimizing or repairing chloroplast functions in response to changing environments. In chloroplasts, the pentatricopeptide-repeat protein GENOMES UNCOUPLED 1 (GUN1) is a master switch that senses aberrant physiological states, such as the photooxidative stress induced by norflurazon (NF) treatment, and represses the expression of photosynthesis-associated nuclear genes (PhANGs). However, it is largely unknown how the retrograde signal is transmitted beyond GUN1. In this study, a protein GUN1-INTERACTING PROTEIN 1 (GIP1), encoded by At3g53630, was identified to interact with GUN1 by different approaches. We demonstrated that GIP1 has both cytosol and chloroplast localizations, and its abundance in chloroplasts is enhanced by NF treatment with the presence of GUN1. Our results suggest that GIP1 and GUN1 may function antagonistically in the retrograde signaling pathway.

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Acknowledgments

We thank Hybrigenics Services for the help with Y2H screening, and Zhong Zhuang for assisting in confocal microscope observations.

Funding

This study was supported by the National Natural Science Foundation of China (Nos. 90817002 and 31770331) and the State Key Basic Research Project of China (No. 2013CB127004).

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Author notes

  1. Xing-Qi Huang, Lin-Juan Wang and Meng-Juan Kong contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China

    Xing-Qi Huang, Lin-Juan Wang, Meng-Juan Kong, Na Huang, Xin-Ya Liu, Han-Yu Liang, Jia-Xin Zhang & Shan Lu

Authors
  1. Xing-Qi Huang
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  2. Lin-Juan Wang
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  3. Meng-Juan Kong
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  4. Na Huang
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  6. Han-Yu Liang
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  7. Jia-Xin Zhang
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  8. Shan Lu
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Contributions

X.H., L.W., M.K., and S.L. designed the research, analyzed results, and wrote the manuscript; X.H., L.W., M.K., N.H., X.-Y.L., H.-Y.L., and J.Z. performed the experiments.

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Correspondence to Shan Lu.

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Huang, XQ., Wang, LJ., Kong, MJ. et al. At3g53630 encodes a GUN1-interacting protein under norflurazon treatment. Protoplasma 258, 371–378 (2021). https://doi.org/10.1007/s00709-020-01578-x

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  • DOI: https://doi.org/10.1007/s00709-020-01578-x

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