Abstract
Aluminum cookware are widely used in many parts of the world. Data is increasing on the leaching of toxic metals from aluminum cookware into food and drink. In the present study, cytogenotoxicity of water boiled in three different aluminum pots (new, 3-year-old, and 6-year-old) in onion root tip’s dividing cells was evaluated using the Allium cepa assay. The concentrations of Pb, As, Cd, Cu, Ni, and Al in the samples were also analyzed. Onion bulbs were grown in the boiled water samples, while tap water served as the control. Cytological and genetic analyses were carried out after 48 h, while analysis of inhibition of root length was carried out after 72 h. The results showed a significant (p < 0.05) cell proliferation and root growth inhibition compared with the control, which is dependent on the duration of use of the aluminum pots. The boiled water samples also caused modification of the root morphology as well as chromosomal aberrations which include sticky chromosomes, anaphase bridge, and disturbed spindle. The highest cytogenotoxicity was observed in the 6-year-old aluminum pot and the least in the new aluminum pot. Pb, As, Cd, Cu, Ni, and Al analyzed in the samples, with the highest concentrations in the 6-year-old aluminum pot, were believed to be responsible for the cytogenotoxicity observed in the A. cepa assay. The data of this study are indications that the aluminum pot–boiled water contains substances with the potential to be cytotoxic and cause mutations in somatic cells of A. cepa.
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Okunola A. Alabi, Sulaimon A. Apata, and Yetunde M. Adeoluwa. The first draft of the manuscript was written by Yetunde M. Adeoluwa and Okunola A. Alabi, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Alabi, O.A., Apata, S.A., Adeoluwa, Y.M. et al. Effect of the duration of use of aluminum cookware on its metal leachability and cytogenotoxicity in Allium cepa assay. Protoplasma 257, 1607–1613 (2020). https://doi.org/10.1007/s00709-020-01536-7
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DOI: https://doi.org/10.1007/s00709-020-01536-7