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Behavior of filamentous temperature-sensitive Z2 (FtsZ2) in the male gametophyte during sexual reproduction processes of flowering plants

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Abstract

Filamentous temperature-sensitive Z (FtsZ) is a critical division protein in bacteria that functions in forming a Z-ring structure to constrict the cell. Since the establishment of the plastid by endosymbiosis of a cyanobacterium into a eukaryotic cell, division via Z-ring formation has been conserved in the plastids of flowering plants. The FtsZ gene was transferred from the cyanobacterial ancestor of plastids to the eukaryotic nuclear genome during evolution, and flowering plants evolved two FtsZ homologs, FtsZ1 and FtsZ2, which are involved in chloroplast division through distinct molecular functions. Regarding the behaviors of FtsZ in nonphotosynthetic cells, the plastid localization of FtsZ1 proteins in the cytoplasm of microspores and pollen vegetative cells but not in generative cells or sperm cells has been reported. On the other hand, the significant accumulation of FtsZ2 transcripts in generative cells has been reported. However, the synthesis of FtsZ2 in the male gamete has not been investigated. Additionally, FtsZ2 behavior has not been analyzed in pollen, a nonphotosynthetic male tissue. Here, we report FtsZ2 protein behaviors in the male gamete by analyzing the localization patterns of GFP-fused protein at various pollen developmental stages and in gametes during the fertilization process. Our results showed that FtsZ2 localization coincided with that of plastids. FtsZ2 protein in male gametes was almost absent, despite the presence of the transcripts. Moreover, transmission of paternal FtsZ2 transcripts to the zygote and endosperm was not observed.

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Acknowledgments

We are grateful to Dr. Miyagishima for providing the AtFtsZ2-1_GFP vector.

Funding

This work was supported by JSPS KAKENHI (Grant Numbers JP17H05832 (to T. I.), JP19H04852 (to T. I.)) and the Strategic Priority Research Promotion Program on Phytochemical Plant Molecular Sciences, Chiba University.

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Authors and Affiliations

  1. Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo-shi, Chiba, 271-8510, Japan

    Yukino Ohashi & Tomoko Igawa

  2. Department of Tropical Medicine and Parasitology, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan

    Toshiyuki Mori

  3. Plant Molecular Science Center, Chiba University, 648 Matsudo, Matsudo-shi, Chiba, 271-8510, Japan

    Tomoko Igawa

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  1. Yukino Ohashi
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  2. Toshiyuki Mori
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  3. Tomoko Igawa
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Correspondence to Tomoko Igawa.

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Ohashi, Y., Mori, T. & Igawa, T. Behavior of filamentous temperature-sensitive Z2 (FtsZ2) in the male gametophyte during sexual reproduction processes of flowering plants. Protoplasma 257, 1201–1210 (2020). https://doi.org/10.1007/s00709-020-01503-2

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  • DOI: https://doi.org/10.1007/s00709-020-01503-2

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