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Stripe rust induced defence mechanisms in the leaves of contrasting barley genotypes (Hordeum vulgare L.) at the seedling stage

  • Prabhjot Singla
  • Rachana D. BhardwajEmail author
  • Simarjit Kaur
  • Jaspal Kaur
Original Article
  • 60 Downloads

Abstract

Puccinia striiformis f. sp. hordei, the causal organism of stripe rust in barley poses serious threats to its production. The present study examined the seedling response and changes in antioxidant defence system along with NADPH oxidase, hydrogen peroxide, and lipid peroxidation marker-malondialdehyde (MDA) in the four barley genotypes namely Jyoti, RD2900, RD2901, and RD2552 in response to M and G-races of stripe rust pathogen. Disease reaction showed Jyoti as susceptible genotype, RD2901 and RD2552 as resistant, whereas RD2900 behaved differentially to both the races. M-race which is predominant was found to be more virulent than G-race of barley stripe rust pathogen. RD2901 showed an increase in activities of NADPH oxidase, catalase, peroxidase, and enzymes of ascorbate-glutathione pathway along with ascorbate and glutathione pool on inoculation with M-race, which was accompanied by the decrease in hydrogen peroxide and MDA contents. Jyoti, on the other hand, showed an increase in peroxidase and glutathione-S-transferase activities only which were unable to maintain redox homeostasis. The scrutiny of data indicated an increase in ASA/DHA ratio on infection in all the genotypes irrespective of their behaviour towards the races. However, GSH/GSSG ratio significantly declined in Jyoti and increased or remained unaffected in the resistant genotypes which suggested that GSH/GSSG might be playing a vital role in imparting tolerance against stripe rust. Further, correlation studies also revealed that leaf damage was positively correlated with H2O2 and MDA contents.

Keywords

Antioxidants defence system Hordeum vulgare L. Redox status Stripe rust 

Abbreviations

CAT

Catalase

GPX

Glutathione peroxidase

GST

Glutathione-S-transferase

MDA

Malondialdehyde

POX

Peroxidase

Notes

Acknowledgements

The authors are thankful to the Department of Plant Breeding and Genetics, Punjab Agricultural University for providing germplasm and assistance in multiplication and maintenance of stripe rust disease under field conditions.

Authors’ contributions

P.S. performed the experiments, analysed the data, wrote the paper and critically revised the manuscript; R.D.B. conceived and designed the experiments, supervised the work with data evaluation and critically revised the manuscript; S.K. and J.K. provided the germplasm and inoculum and supervised the experimental design in the field.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Prabhjot Singla
    • 1
  • Rachana D. Bhardwaj
    • 1
    Email author
  • Simarjit Kaur
    • 2
  • Jaspal Kaur
    • 2
  1. 1.Department of BiochemistryPunjab Agricultural UniversityLudhianaIndia
  2. 2.Department of Plant Breeding and GeneticsPunjab Agricultural UniversityLudhianaIndia

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