Stripe rust induced defence mechanisms in the leaves of contrasting barley genotypes (Hordeum vulgare L.) at the seedling stage
- 60 Downloads
Puccinia striiformis f. sp. hordei, the causal organism of stripe rust in barley poses serious threats to its production. The present study examined the seedling response and changes in antioxidant defence system along with NADPH oxidase, hydrogen peroxide, and lipid peroxidation marker-malondialdehyde (MDA) in the four barley genotypes namely Jyoti, RD2900, RD2901, and RD2552 in response to M and G-races of stripe rust pathogen. Disease reaction showed Jyoti as susceptible genotype, RD2901 and RD2552 as resistant, whereas RD2900 behaved differentially to both the races. M-race which is predominant was found to be more virulent than G-race of barley stripe rust pathogen. RD2901 showed an increase in activities of NADPH oxidase, catalase, peroxidase, and enzymes of ascorbate-glutathione pathway along with ascorbate and glutathione pool on inoculation with M-race, which was accompanied by the decrease in hydrogen peroxide and MDA contents. Jyoti, on the other hand, showed an increase in peroxidase and glutathione-S-transferase activities only which were unable to maintain redox homeostasis. The scrutiny of data indicated an increase in ASA/DHA ratio on infection in all the genotypes irrespective of their behaviour towards the races. However, GSH/GSSG ratio significantly declined in Jyoti and increased or remained unaffected in the resistant genotypes which suggested that GSH/GSSG might be playing a vital role in imparting tolerance against stripe rust. Further, correlation studies also revealed that leaf damage was positively correlated with H2O2 and MDA contents.
KeywordsAntioxidants defence system Hordeum vulgare L. Redox status Stripe rust
The authors are thankful to the Department of Plant Breeding and Genetics, Punjab Agricultural University for providing germplasm and assistance in multiplication and maintenance of stripe rust disease under field conditions.
P.S. performed the experiments, analysed the data, wrote the paper and critically revised the manuscript; R.D.B. conceived and designed the experiments, supervised the work with data evaluation and critically revised the manuscript; S.K. and J.K. provided the germplasm and inoculum and supervised the experimental design in the field.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Ali M, Cheng Z, Ahmad H, Hayat S (2018) Reactive oxygen species (ROS) as defences against a broad range of plant fungal infections and case study on ROS employed by crops against Verticillium dahliae wilts. J Plant Interact 13:353–363. https://doi.org/10.1080/17429145.2018.1484188 CrossRefGoogle Scholar
- Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY (2015) Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage. Front Plant Sci 6:1–11. https://doi.org/10.3389/fpls.2015.00779 CrossRefGoogle Scholar
- Fernandes LDR, Bonaldo SM, de Jesus Rodrigues D, Vieira-Junior GM, Schwan-Estrada KRF, da Silva CR, Vercosa AGA, de Oliveira DL, Debias BW (2019) Induction of phytoalexins and proteins related to pathogenesis in plants treated with extracts of cutaneous secretions of southern Amazonian Bufonidae amphibians. PLoS One 14:e0211020. https://doi.org/10.1371/journal.pone.0211020 CrossRefGoogle Scholar
- Heller J, Tudzynski P (2011) Reactive oxygen species in phytopathogenic fungi: signaling, development, and disease. Annu Rev Phytopathol 49:369–390. https://doi.org/10.1146/annurev-phyto-072910-095355 CrossRefGoogle Scholar
- Hossain MA, Asada K (1984) Purification of dehydroascorbate reductase from spinach and its characterization as a thiol enzyme. Plant Cell Physiol 25:85–92. https://doi.org/10.1093/oxfordjournals.pcp.a076700 CrossRefGoogle Scholar
- Hossain MA, Nakano Y, Asada K (1984) Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 25:385–395. https://doi.org/10.1093/oxfordjournals.pcp.a076726 CrossRefGoogle Scholar
- Kharub AS, Kumar D, Singh J, Kumar L, Kumar V, Khippal A, Kumar S, Kumar RS, Bhardwaj SC, Katare S, Malik R, Verma A, Sharma I (2014–2015) All India coordinated wheat and barley improvement project. Progress Rep VI:3.1–3.47 Indian Institute of Wheat and Barley Research, KarnalGoogle Scholar
- Kumar J, Singh DC, Singh AP, Verma SK (2013) Screening of chickpea genotypes for resistant against POX borer Helicoverpa armigera Hubn. Trends Biosci 6:101–103Google Scholar
- Kumbhakar DV, Dattaa AK, Dasa D, Ghosha B, Pramanika A, Gupta S (2019) Assessment of oxidative stress, antioxidant enzyme activity and cellular apoptosis in a plant based system (Nigella sativa L.; black cumin) induced by copper and cadmium sulphide nanomaterials. Environ Nanotechnol Monit Manage 11:100196. https://doi.org/10.1016/j.enmm.2018.100196 CrossRefGoogle Scholar
- Marklund S, Marklund G (1974) Involvement of superoxide anion radical in the autoxidation of pyragallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:169–174. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x CrossRefGoogle Scholar
- Mbengue M, Navaud O, Peyraud R, Barascud M, Badet T, Vincent R, Barbacci A, Raffaele S (2016) Emerging trends in molecular interactions between plants and the broad host range fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum. Front Plant Sci 7:422. https://doi.org/10.3389/fpls.2016.00422 CrossRefPubMedPubMedCentralGoogle Scholar
- McNeal FH, Konzak CF, Smith EP, Tate WS, Russel TS (1971) A uniform system for recording and processing cereal research data. US Agri Res SerGoogle Scholar
- Mollayi S, Farzaneh M, Ghanati F, Aboul-Enein HY, Ghassempour A (2016) Study of catechin, epicatechin and their enantiomers during the progression of witches’ broom disease in Mexican lime (Citrus aurantifolia). Physiol Mol Plant 93:93–98. https://doi.org/10.1016/j.pmpp.2015.12.002 CrossRefGoogle Scholar
- Nakano Y, Asada K (1987) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880. https://doi.org/10.1093/oxfordjournals.pcp.a076232 CrossRefGoogle Scholar
- Rodriguez-Serrano M, Romero-Puertas MC, Sanz-Fernandez M, Hu J, Sandalio LM (2016) Peroxisomes extend peroxules in a fast response to stress via a reactive oxygen species-mediated induction of the peroxin PEX11a. Plant Physiol 171:1665–1674. https://doi.org/10.1104/pp.16.00648 CrossRefPubMedPubMedCentralGoogle Scholar
- Shannon LM, Kay E, Lew JK (1966) Peroxidase isoenzyme from horseradish roots. Isolation and physical properties. J Biol Chem 241:2166–2172Google Scholar
- Siddique Z, Akhtar KP, Hameed A, Sarwar N, Imran-Ul-Haq KSA (2014) Biochemical alterations in leaves of resistant and susceptible cotton genotypes infected systemically by cotton leaf curl Burewala virus. J Plant Interact 9:702–711. https://doi.org/10.1080/17429145.2014.905800 CrossRefGoogle Scholar
- Yalcinkaya T, Uzilday B, Ozgur R, Turkan I (2019) The roles of reactive carbonyl species in induction of antioxidant defence and ROS signalling in extreme halophytic model Eutrema parvulum and glycophytic model Arabidopsis thaliana. Environ Exp Bot 160:81–91. https://doi.org/10.1016/j.envexpbot.2019.01.009 CrossRefGoogle Scholar
- Zeng X, Guo Y, Qijun Xu Q, Mascher M, Guo G, Li S, Mao L, Liu Q, Xia Z, Zhou J, Yuan H, Tai S, Wang Y, Wei Z, Song L, Zha S, Li S, Tang Y, Bai L, Zhuang Z, He W, Zhao S, Fang X, Gao Q, Yin Y, Wang J, Yang H, Zhang J, Henry RJ, Stein N, Tashi N (2018) Origin and evolution of qingke barley in Tibet. Nature Comm 9:1–11. https://doi.org/10.1038/s41467-018-07920-5 CrossRefGoogle Scholar