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Grafting improves tomato drought tolerance through enhancing photosynthetic capacity and reducing ROS accumulation

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Abstract

Drought is the main meteorological threat to plants and limits plant growth, development, and adaptation to environmental changes. However, root-shoot communication plays a vital role in improving tomato plant drought tolerance, especially when cultivars are grafted onto drought-tolerant rootstock. In this study, the relationship between photosynthetic capacity and reactive oxygen species (ROS) in response to drought stress was studied in tomato grafted with different drought-resistant tomato seedlings. To determine the drought-relieving effect of drought-tolerant rootstocks, we measured the effects of grafting on plant growth, net photosynthetic rate (Pn), ROS accumulation, and antioxidant enzyme activities in tomato leaves and roots under drought stress. Plant growth and Pn were significantly inhibited by drought, but ROS accumulation and antioxidant enzyme activities were significantly increased. Treatment with drought-tolerant tomato seedlings significantly increased plant growth and increased Pn under water-deficit conditions compared with those grafted with drought-susceptible rootstock. In addition, the plants grafted with drought-tolerant seedlings had increased activities of partial antioxidant enzymes, leading to decreased ROS production. Our results indicate that tomato grafted with drought-tolerant seedlings alleviated the phytotoxicity and oxidative damage caused by drought by regulating antioxidant enzymes under drought stress.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

H2O2 :

Hydrogen peroxide

Fv/Fm:

maximal photochemical efficiency

Fv′/Fm′:

Effective quantum use efficiency of (photosystem II) PSII in the light-adapted state

MA:

Malic acid

MDA:

Malondialdehyde

O :

Superoxide anion

Pn:

Net photosynthetic rate

POD:

Peroxidase

PSII:

Photosystem II

REL:

Relative electrolyte leakage

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

Tr:

Transpiration rate

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Funding

This work was supported by the Special Foundation for Modern Agricultural Industry Technology System of Shandong Province (no. SDAIT-05-05) and the Double First-Class Discipline Construction Project of Shandong Province (no. SYL2017YSTD06).

Author information

Zhihuan Zhang and Kun Xu designed the experiments; Zhihuan Zhang, Bili Cao, and Song Gao performed the experiments; and Zhihuan Zhang and Kun Xu wrote the manuscript.

Correspondence to Kun Xu.

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The authors declare that they have no conflict of interest.

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Zhang, Z., Cao, B., Gao, S. et al. Grafting improves tomato drought tolerance through enhancing photosynthetic capacity and reducing ROS accumulation. Protoplasma 256, 1013–1024 (2019). https://doi.org/10.1007/s00709-019-01357-3

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Keywords

  • Tomato
  • Grafting
  • Drought tolerance
  • Photosynthetic
  • ROS accumulation
  • Antioxidant