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Protoplasma

, Volume 255, Issue 5, pp 1413–1422 | Cite as

Selection and regeneration of Vitis vinifera Chardonnay hydroxyproline-resistant calli

  • Chaoxia Wang
  • Rongrong He
  • Jiang Lu
  • Yali Zhang
Original Article

Abstract

Proline (Pro) accumulation protects plant cell under abiotic stress. Hydroxyproline (Hyp) as selection agent is a toxic analog of proline and promotes Pro overaccumulation. In this study, Chardonnay calli were firstly irradiated with different dosages of 60Co and then cultured on a Hyp-added medium. Finally, some stable hydroxyproline-resistant (HR) calli were obtained. When calli were cultured on 4 mM Hyp medium for 7 days, intracellular Pro content of the HR calli was five times higher than that detected in the normal calli. The regeneration of HR calli into plantlets was much slower than that of normal ones. When cultured on woody plant medium (WPM) containing 10 mM NaCl for 14 days, HR plantlets still grew well with lower Pro than withered normal plantlets. qRT-PCR results of Pro biosynthesis-related genes in HR plantlets showed that three genes VvP5CS, VvOAT, and VvP5CDH were conducive for Pro accumulation. These results confirmed that HR plantlets acquired salt tolerance ability. We prospect that this procedure to obtain salt-tolerant plants may be valuable to breed programs and improve grapevine genotypes with increased tolerance to salt and other abiotic stresses.

Keywords

Grapevine Proline Hydroxyproline Salt stress 

Abbreviations

Hyp

Hydroxyproline

HR

Hydroxyproline-resistant

OAT

Orn-δ-amino-transferase

Pro

Proline

P5C

1-Pyrroline-5-carboxylate

P5CS

1-Pyrroline-5-carboxylate synthase

P5CR

1-Pyrroline-5-carboxylate reductase

ProDH

Pro dehydrogenase

P5CDH

P5C dehydrogenase

Notes

Acknowledgments

We are grateful to the following for providing Vitis vinifera Chardonnay calli for this study: X. Xia at Center for Viticulture and Small Fruit Research, Florida A&M University.

Funding

This study was supported by the earmarked fund for China Agriculture Research System (Grant No. CARS-29-yc-2) and Guangxi Bagui Scholar Fund (2013-3).

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Chaoxia Wang
    • 1
  • Rongrong He
    • 1
  • Jiang Lu
    • 2
  • Yali Zhang
    • 1
  1. 1.The Viticulture and Enology Program, College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingPeople’s Republic of China
  2. 2.Center for Viticulture and Enology, School of Agriculture BiologyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China

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