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Overexpression of AlTMP2 gene from the halophyte grass Aeluropus littoralis in transgenic tobacco enhances tolerance to different abiotic stresses by improving membrane stability and deregulating some stress-related genes

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Abstract

Herein, we report isolation of the AlTMP2 gene from the halophytic C4 grass Aeluropus littoralis. The subcellular localization suggested that AlTMP2 is a plasma membrane protein. In A. littoralis exposed to salt and osmotic stresses, the AlTMP2 gene was induced early and at a high rate, but was upregulated relatively later in response to abscisic acid and cold treatments. Expression of AlTMP2 in tobacco conferred improved tolerance against salinity, osmotic, H2O2, heat, and freezing stresses at the germination and seedling stages. Under control conditions, no growth or yield penalty were mentioned in transgenic plants due to the constitutive expression of AlTMP2. Interestingly, under greenhouse conditions, the seed yield of transgenic plants was significantly higher than that of non-transgenic (NT) plants grown under salt or drought stress. Furthermore, AlTMP2 plants had less electrolyte leakage, higher membrane stability, and lower Na+ and higher K+ accumulation than NT plants. Finally, six stress-related genes were shown to be deregulated in AlTMP2 plants relative to NT plants under both control and stress conditions. Collectively, these results indicate that AlTMP2 confers abiotic stress tolerance by improving ion homeostasis and membrane integrity, and by deregulating certain stress-related genes.

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Acknowledgments

This work was funded by The National Plan for Science Technology and Innovation, http://npst.ksu.edu.sa/en, (NPSTI, Project No. 11-Bio1828-02) in the Kingdom of Saudi Arabia. The authors are grateful to the Deanship of Scientific Research, King Saud University and Agricultural Research Center, College of Food and Agriculture Sciences. The authors thank the Visiting Professor Program of King Saud University Saudi Arabia and the Agropolis Foundation under the REFUGE platform, CIRAD-Montpellier France, for encouraging this collaborative work.

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  1. Walid Ben-Romdhane & Afif Hassairi

    Present address: Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia

  2. Walid Ben-Romdhane and Afif Hassairi contributed equally to this work.

Authors and Affiliations

  1. Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia

    Abdullah Al-Doss

  2. Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, University of Sfax, B.P 1177, 3018, Sfax, Tunisia

    Walid Ben-Romdhane, Rania Ben-Saad, Nabil Zouari, Ali Mahjoub & Afif Hassairi

  3. CIRAD-UMR AGAP (Centre de coopération Internationale en Recherche Agronomique pour le Développement), Avenue Agropolis, 34398, Montpellier Cedex 5, France

    Donaldo Meynard & Emmanuel Guiderdoni

  4. Laboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Sciences of Sfax, University of Sfax, B.P 802, 3038, Sfax, Tunisia

    Lotfi Fki

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Ben-Romdhane, W., Ben-Saad, R., Meynard, D. et al. Overexpression of AlTMP2 gene from the halophyte grass Aeluropus littoralis in transgenic tobacco enhances tolerance to different abiotic stresses by improving membrane stability and deregulating some stress-related genes. Protoplasma 255, 1161–1177 (2018). https://doi.org/10.1007/s00709-018-1223-3

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