Protoplasma

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Congruence between PM H+-ATPase and NADPH oxidase during root growth: a necessary probability

Original Article
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Abstract

Plasma membrane (PM) H+-ATPase and NADPH oxidase (NOX) are two key enzymes responsible for cell wall relaxation during elongation growth through apoplastic acidification and production of ˙OH radical via O2˙, respectively. Our experiments revealed a putative feed-forward loop between these enzymes in growing roots of Vigna radiata (L.) Wilczek seedlings. Thus, NOX activity was found to be dependent on proton gradient generated across PM by H+-ATPase as evident from pharmacological experiments using carbonyl cyanide m-chlorophenylhydrazone (CCCP; protonophore) and sodium ortho-vanadate (PM H+-ATPase inhibitor). Conversely, H+-ATPase activity retarded in response to different ROS scavengers [CuCl2, N, N’ –dimethylthiourea (DMTU) and catalase] and NOX inhibitors [ZnCl2 and diphenyleneiodonium (DPI)], while H2O2 promoted PM H+-ATPase activity at lower concentrations. Repressing effects of Ca+2 antagonists (La+3 and EGTA) on the activity of both the enzymes indicate its possible mediation. Since, unlike animal NOX, the plant versions do not possess proton channel activity, harmonized functioning of PM H+-ATPase and NOX appears to be justified. Plasma membrane NADPH oxidase and H+-ATPase are functionally synchronized and they work cooperatively to maintain the membrane electrical balance while mediating plant cell growth through wall relaxation.

Keywords

Calcium NADPH oxidase (NOX) Plasma membrane (PM) H+-ATPase Proton gradient Root growth ROS 

Notes

Acknowledgements

One of the authors (AM) gratefully recognizes financial support for the present investigation from University Grants Commission (UGC), New Delhi, India, as BSR Fellowship [vide letter F. No. 25-1/2014-15(BSR)/220/2009/(BSR)].

Author contributions

RKK envisaged the study. AM and RKK designed the work. AM performed the experiments. AM and RKK wrote the article.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

709_2018_1217_MOESM1_ESM.docx (3.4 mb)
ESM 1 (DOCX 3505 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Plant Physiology and Biochemistry Laboratory, Department of BotanyVisva-Bharati UniversitySantiniketanIndia
  2. 2.Department of BotanyCity CollegeKolkataIndia

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