Abstract
The cells in the foliose thallus of trebouxiophycean alga Prasiola japonica apparently develop into 2 × 2 cell groups composed of two two-celled groups, each of which is a pair of derivative cells of the latest cell division. In the present study, the structural features of cell walls of the alga P. japonica concerning the formation of the cell groups were investigated using histochemical methods. Thin cell layers stained by Calcofluor White appeared to envelope the two-celled and four-celled groups separately and, hence, separated them from neighboring cell groups, and the Calcofluor White-negative gaps between neighboring four-celled groups were specifically stained by lectins, such as soybean agglutinin, jacalin, and Vicia villosa lectin conjugated with fluorescein. These results indicated that the Calcofluor White-positive cell wall layer of parent cell that existed during two successive cell divisions structurally distinguished two-celled and four-celled groups from others in this alga. Moreover, the results suggested that the cell wall components of the Calcofluor White-negative gaps would possibly contribute to the formation of the planar thallus through lateral union of the cell groups.
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Notes
The culture strain was deposited in the Microbial Culture Collection at the National Institute for Environmental Studies (Tsukuba, Japan; strain no. NIES-3960).
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Acknowledgements
We are grateful to Dr. Maki Yamamoto, Senshu University, Japan, for her contribution to the ultrastructural research of Trebouxophycean algae and critical advices on the discussion in the present study. We also thank the Shimanto Genryu Center, Tsuno Town, Kochi, Japan, for providing kind support during the collection of P. japonica.
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Mine, I., Kinoshita, U., Kawashima, S. et al. Cell wall layers delimit cell groups derived from cell division in the foliose trebouxiophycean alga Prasiola japonica. Protoplasma 255, 1253–1256 (2018). https://doi.org/10.1007/s00709-018-1206-4
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DOI: https://doi.org/10.1007/s00709-018-1206-4