A review of “tethers”: elastic connections between separating partner chromosomes in anaphase
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Recent work has demonstrated the existence of elastic connections, or tethers, between the telomeres of separating partner chromosomes in anaphase. These tethers oppose the poleward spindle forces in anaphase. Functional evidence for tethers has been found in a wide range of animal taxa, suggesting that they might be present in all dividing cells. An examination of the literature on cell division from the nineteenth century to the present reveals that connections between separating partner chromosomes in anaphase have been described in some of the earliest observations of cell division. Here, we review what is currently known about connections between separating partner chromosomes in anaphase, and we speculate on possible functions of tethers, and on what they are made of and how one might determine their composition.
KeywordsMitosis Meiosis Anaphase Chromosomes Interzonal connections Tethers
LP was funded by a Scadden Fellowship through the Bucknell University. AF was funded by a grant from the Natural Sciences and Engineering Research Council of Canada.
Compliance with ethical standards
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- Andrews FM (1915) Die Wirkung der Zentrifugalkraft auf Pflanzen. Jahrb Wiss Bot 56:221–253Google Scholar
- Carnoy J (1885) La cytodiérèse ches les Arthropodes. Aug Peeters Libraire, LouvainGoogle Scholar
- Fabian L, Xia X, Venkitaramani DV, Johansen KM, Johansen J, Andrew DJ, Forer A (2007a) Titin in insect spermatocyte spindle fibers associates with microtubules, actin, myosin and the matrix proteins skeletor, megator and chromator. J Cell Sci 120(13):2190–2204. https://doi.org/10.1242/jcs.03465 CrossRefPubMedGoogle Scholar
- Hughes AF, Swann MM (1948) Anaphase movements in the living cell. J Exp Biol 25:45–72Google Scholar
- Inoue S (1952) The effect of colchicine on the microscopic and submicroscopic structure of the mitotic spindle. Exp Cell Res Suppl 2:305–318Google Scholar
- McIntosh JR, Hays T (2016) A brief history of research on mitotic mechanisms. Biology 5(4). https://doi.org/10.3390/biology5040055
- Montgomery TH (1899) The spermatogenesis in Pentatoma up to the formation of the spermatid. Zool Jahrb Abt f Anat 12:1–88Google Scholar
- Schrader F (1944) Mitosis. Columbia University Press, New YorkGoogle Scholar
- Sillers PJ, Forer A (1981) Analysis of chromosome movement in crane fly spermatocytes by ultraviolet microbeam irradiation of individual chromosomal spindle fibres. II. Action spectra for stopping chromosome movement and for blocking ciliary beating and myofibril contractions. Canadian J Biochem 59:777–792CrossRefGoogle Scholar
- Spurck T, Forer A, Pickett-Heaps J (1997) Ultraviolet microbeam irradiations of epithelial and spermatocyte spindles suggest that forces act on the kinetochore fibre and are not generated by its disassembly. Cell Motil Cytoskel 36(2):136–148. https://doi.org/10.1002/(SICI)1097-0169(1997)36:2<136::AID-CM4>3.0.CO;2-7 CrossRefGoogle Scholar
- Stevens NM (1905) Studies in spermatogenesis with especial reference to the “accessory chromosome”. Carnegie Institution of Washington Publication 36:1–33Google Scholar
- Swann MM (1951) Protoplasmic structure and mitosis. I. The birefringence of the metaphase spindle and asters of the living sea urchin egg. J Exp Biol 28:417–433Google Scholar
- Swann MM, Mitchison JM (1953) Cleavage of sea-urchin eggs in colchicine. J Exp Biol 30:506–514Google Scholar
- Walker DL, Wang D, Jin Y, Rath U, Wang Y, Johansen J, Johansen KM (2000) Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix. J Cell Biol 151(7):1401–1411. https://doi.org/10.1083/jcb.151.7.1401 CrossRefPubMedPubMedCentralGoogle Scholar