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Protoplasma

, Volume 255, Issue 3, pp 761–771 | Cite as

Responses of He-Ne laser on agronomic traits and the crosstalk between UVR8 signaling and phytochrome B signaling pathway in Arabidopsis thaliana subjected to supplementary ultraviolet-B (UV-B) stress

  • Limei Gao
  • Yongfeng Li
  • Zhihua Shen
  • Rong Han
Original Article
  • 285 Downloads

Abstract

UV-B acclimation effects and UV-B damage repair induced by a 632.8-nm He-Ne laser were investigated in Arabidopsis thaliana plants in response to supplementary UV-B stress. There was an increasing trend in growth parameters in the combination-treated plants with He-Ne laser and UV-B light compared to those stressed with enhanced UV-B light alone during different developmental stages of plants. The photosynthetic efficiency (Pn) and survival rates of seedlings were significantly higher in the combination treatments than UV-B stress alone. The expression of UVR8, phytochrome B (PhyB), and their mediated signal responsive genes such as COP1, HY5, and CHS were also significantly upregulated in plants with the laser irradiation compared with other groups without the laser. Levels of flavonol accumulation in leaves and capsule yield of He-Ne laser-treated plants were increased. The phyB-9 mutants were more sensitive to enhanced UV-B stress and had no obvious improvements in plant phenotypic development and physiological damage caused by enhanced UV-B stress after He-Ne laser irradiation. Our results suggested that UVR8 and its mediated signaling pathway via interaction with COP1 can be induced by He-Ne laser, and these processes were dependent on cytoplasmic PhyB levels in plant cells, which might be one of the most important mechanisms of He-Ne laser on UV-B protection and UV-B damage repair. These current data have also elucidated that the biostimulatory effects of He-Ne laser on Arabidopsis thaliana plants would happen not only during the early growth stage but also during the entire late developmental stage.

Keywords

UV-B protection and acclimation Arabidopsis thaliana He-Ne laser UVR8 Phytochrome B 

Notes

Acknowledgements

We thank Dr. Shaoying Zhang (Oregon State University, USA) for critical reading and editing the manuscript, and we acknowledge the foundation of Higher School Science and Technology Innovation Project of Shanxi Province (Grant no. 20161106) and Cultivation Project of Youth Backbone Teacher of Shanxi Normal University.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  • Limei Gao
    • 1
    • 2
    • 3
  • Yongfeng Li
    • 3
    • 4
  • Zhihua Shen
    • 2
    • 3
  • Rong Han
    • 2
    • 3
  1. 1.Modern College of Humanities and SciencesShanxi Normal UniversityLinfenChina
  2. 2.College of Life ScienceShanxi Normal UniversityLinfenChina
  3. 3.Higher Education Key Laboratory of Plant Molecular and Environmental Stress ResponseShanxi Normal UniversityLinfenChina
  4. 4.Analysis and Testing CenterShanxi Normal UniversityLinfenChina

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