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Structure of the style and pollen tube pathway in the Ziziphoid and Rhamnoid clades of Rhamnaceae

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Abstract

The ultrastructure of the style and pollen tube pathway before, during and after anthesis were studied in 13 species belonging to the tribes Pomaderreae, Paliureae, Colletieae and Gouanieae (Ziziphoid clade) and Rhamneae (Rhamnoid clade) using light microscopy and transmission electron microscopy. The aim of this study is to provide new morphological characters useful for phylogenetic analysis at suprageneric level in Rhamnaceae. The patterns of pollen tube growth and the ultrastructural changes undergone by cells of the style were also described. Species of Rhamneae (Scutia buxifolia and Condalia buxifolia) have a solid style, with the transmitting tissue forming three independent strands (S. buxifolia) or a central, single horseshoe-shaped strand as seen in transversal section (C. buxifolia) which could derive from the fusion of formerly independent strands. In contrast, Pomaderreae, Gouanieae and Paliureae showed semi-solid styles, while in Colletieae, as previously reported, the style is hollow with two or three stylar canals. The style anatomy and the ultrastructure of the pollen tube pathway show that there is a tendency towards a solid style with a single strand of transmitting tissue within the family. The three-canalled hollow style could be the plesiomorphic state of the character “type of style” in the family, the semi-solid style the synapomorphic state and the solid style with three strands of transmitting tissue the apomorphic state, with the solid style with a single strand of transmitting tissue as the most derived state. Therefore, Colletieae would be the most basal tribe of the Ziziphoid clade.

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Funding

This research was financially supported by a grant (UBACyT 2013–2016 GC 20020120100056BA) to B. Galati. M. Gotelli and D. Medan are affiliated with CONICET, Argentina.

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Authors and Affiliations

  1. Facultad de Agronomía, Departamento de Recursos Naturales y Ambiente, Universidad de Buenos Aires, Cátedra de Botánica General. Av. San Martín 4453 (1417), Buenos Aires, Argentina

    Marina M. Gotelli, Beatriz G. Galati, Gabriela Zarlavsky & Diego Medan

  2. CONICET, Buenos Aires, Argentina

    Marina M. Gotelli & Diego Medan

Authors
  1. Marina M. Gotelli
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  2. Beatriz G. Galati
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  3. Gabriela Zarlavsky
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  4. Diego Medan
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Correspondence to Marina M. Gotelli.

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Gotelli, M.M., Galati, B.G., Zarlavsky, G. et al. Structure of the style and pollen tube pathway in the Ziziphoid and Rhamnoid clades of Rhamnaceae. Protoplasma 255, 501–515 (2018). https://doi.org/10.1007/s00709-017-1167-z

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  • DOI: https://doi.org/10.1007/s00709-017-1167-z

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