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Molecular characterisation of a DREB gene from Sophora moorcroftiana, an endemic species of plateau

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Abstract

Various plant species in the Qinghai-Tibet Plateau exposed to harsh conditions, such as low oxygen, drought, extremely low temperatures and salinity, have evolved both molecular and physiological adaptation strategies to deal with these multiple stresses. Sophora moorcroftiana (Benth.) Baker (Fabaceae) is a highly drought-resistant endemic Sophora shrub species in the Qinghai-Tibet Plateau. In our previous study, a drought-induced DREB transcription factor gene was identified and was designated as SmDREB1. SmDREB1-GFP fusion construct was introduced into Arabidopsis protoplast to characterise the function of SmDREB1 in drought resistance. The results showed that SmDREB1 targets the nucleus of Arabidopsis protoplast. Ectopic expression of SmDREB1 in model plant species Arabidopsis was performed. The transgenic lines showed increasing expressions of drought marker genes including AtDHN, AtLEA, AtPIP2 ;2, AtPIP2;3 and AtRD29, increasing activities of antioxidant enzymes and proline contents and increasing light-use efficiency under drought stress as compared with the wild-type plants; SmDREB1 transgenic lines are more resistant to drought than wild-type plants. Therefore, the SmDREB1 is a drought-resistant transcription factor gene of S. moorcroftiana and could be a candidate in genetic engineering to improve drought resistance of plateau plant species.

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (31260189).

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Authors and Affiliations

  1. College of Agriculture, Guizhou University, Guiyang, 550025, People’s Republic of China

    Huie Li

  2. College of Forestry, Guizhou University, Guiyang, 550025, People’s Republic of China

    Qiqiang Guo

  3. Agricultural and Animal Husbandry College, Tibet University, Nyingchi, 860000, People’s Republic of China

    Huie Li, Yanfu Zhang, Qiqiang Guo & Weijie Yao

  4. Forest Inventory and Planning Institute of Tibet Autonomous Region, Lhasa, 850000, People’s Republic of China

    Weijie Yao

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  1. Huie Li
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  2. Yanfu Zhang
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  3. Qiqiang Guo
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  4. Weijie Yao
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Correspondence to Huie Li.

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Handling Editor: Bhumi Nath Tripathi

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Li, H., Zhang, Y., Guo, Q. et al. Molecular characterisation of a DREB gene from Sophora moorcroftiana, an endemic species of plateau. Protoplasma 254, 1735–1741 (2017). https://doi.org/10.1007/s00709-016-1065-9

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  • DOI: https://doi.org/10.1007/s00709-016-1065-9

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