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Characterization of a bacterioferritin comigratory protein family 1-Cys peroxiredoxin from Candidatus Liberibacter asiaticus

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Abstract

To defend against the lethality of the reactive oxygen species (ROS), nature has armed microorganisms with a range of antioxidant proteins. These include peroxiredoxin (Prx) super family proteins which are ubiquitous cysteine-based non-heme peroxidases. The phytopathogenic bacterium Candidatus Liberibacter asiaticus (CLA), an etiological agent of citrus plants diseases, posses many genes for defense against oxidative stress. The bacterioferritin comigratory protein (BCP), a member of Prxs, is part of an oxidative stress defense system of CLA. The key residue of these enzymes is peroxidatic Cys (termed CPSH) which is contained within an absolutely conserved PXXX (T/S) XXC motif. In the present study, a 1-Cys Prx enzyme (CLa-BCP), having CPSH/sulfenic acid cysteine (C-46) but lacking the resolving cysteine (CRSH), was characterized from CLA. The peroxidase activity was demonstrated using a non-physiological electron donor DTT against varied substrates. The protein was shown to have the defensive role against peroxide-mediated cell killing and an antioxidant activity. In vitro DNA-binding studies showed that this protein can protect supercoiled DNA from oxidative damage. To the best of our knowledge, this is the first report on a 1-Cys BCPs to have an intracellular reactive oxygen species scavenging activity.

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Acknowledgements

This work was supported by a grant no. BT/PR9877/BRB/10/1274/2014 from the Department of Biotechnology, Ministry of Science and Technology, Government of India. The CD and Fluorescence studies were performed in NMR facility at Institute Instrumentation Centre (IIC), IIT Roorkee. AS and NK thanks the Department of Biotechnology (DBT), Government of India for fellowship. The authors thank Dr. P. Selva Kumar for scientific discussion.

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  1. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India

    Anamika Singh, Narender Kumar, Prabhat P. S. Tomar, Partha Roy & Ashwani K. Sharma

  2. Plant Virology Laboratory, ICAR—Central Citrus Research Institute, Nagpur, 440 010, India

    Sumit Bhose & Dilip Kumar Ghosh

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  1. Anamika Singh
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  2. Narender Kumar
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Correspondence to Ashwani K. Sharma.

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Singh, A., Kumar, N., Tomar, P.P.S. et al. Characterization of a bacterioferritin comigratory protein family 1-Cys peroxiredoxin from Candidatus Liberibacter asiaticus. Protoplasma 254, 1675–1691 (2017). https://doi.org/10.1007/s00709-016-1062-z

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