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Chloroplast differentiation in the growing leaves of Arabidopsis thaliana


Here, we describe the development of chloroplasts and the buildup of the thylakoid membranes in growing Arabidopsis leaves. Organelles were analyzed from three distinct positions, namely, at the tip, the upper leaf margin, and the base from leaves 1, 3, 5, and 7 of 14-day-old plants. Clear developmental gradients are described within a given leaf and between leaves of different age. Chloroplasts at the tip of every leaf are always the most matured within a given leaf, while already at the upper leaf margin a differentiation gradient can be observed from the edge of the leaf toward the midrib. The data presented here can serve as a standard for a subcellular phenotypic analysis in chloroplast biogenesis mutants.

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This paper is dedicated to the late Prof. P. Sitte University of Freiburg. We gratefully acknowledge the invaluable ongoing discussion and scientific support by Prof. G. Schultz. This work was in parts financed by the Deutsche Forschungsgemeinschaft Cluster of Excellence CIPSM.

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Correspondence to Jürgen Soll.

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Gügel, I.L., Soll, J. Chloroplast differentiation in the growing leaves of Arabidopsis thaliana . Protoplasma 254, 1857–1866 (2017).

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  • Transmission electron microscopy
  • Thylakoids
  • Leaf development
  • Chloroplast differentiation
  • Arabidopsis thaliana