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Cytomixis in tobacco microsporogenesis: are there any genome parts predisposed to migration?

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Abstract

Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization (FISH), and the question whether cytomixis is a random or a targeted process is answered. The distribution of four repetitive sequences used for identifying the tobacco chromosomes—NTRS, 5S rDNA, GRS, and HSR60—has been examined in the migrating chromatin, and the micronuclei formed after cytomixis. The distribution of tobacco S and T genomes has been analyzed in the cytomictic chromatin using genomic in situ hybridization (GISH). As has been shown (χ 2 test), the labeled DNA probes marking the listed sequences in tobacco genome are observed in the micronuclei formed after cytomixis with the probability not exceeding the theoretically expected value if cytomixis considered as a random process. Thus, it is shown that cytomixis is not a targeted process, and the chromosomes migrate between microsporocytes in a random manner.

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Acknowledgments

The work was supported by the Russian Foundation for Basic Research (grant no. 16-34-60007 mol_a_dk) and Siberian Branch of the Russian Academy of Science under the program “Genetic platform for performing plant selection tasks: basic and applied research” (0324-2015-0005). The microscopy was conducted at the Joint Access Center for Microscopy of Biological Objects with the Siberian Branch of the Russian Academy of Sciences.

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  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk, 630090, Russian Federation

    Sergey Mursalimov & Elena Deineko

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  1. Sergey Mursalimov
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  2. Elena Deineko
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Correspondence to Sergey Mursalimov.

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The authors declare that they have no conflict of interest.

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Handling Editor: Benedikt Kost

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Mursalimov, S., Deineko, E. Cytomixis in tobacco microsporogenesis: are there any genome parts predisposed to migration?. Protoplasma 254, 1379–1384 (2017). https://doi.org/10.1007/s00709-016-1028-1

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  • DOI: https://doi.org/10.1007/s00709-016-1028-1

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