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Protoplasma

, Volume 254, Issue 2, pp 685–696 | Cite as

SmLEA2, a gene for late embryogenesis abundant protein isolated from Salvia miltiorrhiza, confers tolerance to drought and salt stress in Escherichia coli and S. miltiorrhiza

  • Huaiqin Wang
  • Yucui Wu
  • Xinbing Yang
  • Xiaorong Guo
  • Xiaoyan CaoEmail author
Original Article

Abstract

Abiotic stresses, such as drought and high salinity, are major factors that limit plant growth and productivity. Late embryogenesis abundant (LEA) proteins are members of a diverse, multigene family closely associated with tolerance to abiotic stresses in numerous organisms. We examined the function of SmLEA2, previously isolated from Salvia miltiorrhiza, in defense responses to drought and high salinity. Phylogenetic analysis indicated that SmLEA2 belongs to the LEA_2 subfamily. Its overexpression in Escherichia coli improved growth performance when compared with the control under salt and drought stresses. We further characterized its roles in S. miltiorrhiza through overexpression and RNAi-mediated silencing. In response to drought and salinity treatments, transgenic plants overexpressing SmLEA2 exhibited significantly increased superoxide dismutase activity, reduced levels of lipid peroxidation, and more vigorous growth than empty-vector control plants did. However, transgenic lines in which expression was suppressed showed the opposite results. Our data demonstrate that SmLEA2 plays an important role in the abiotic stress response and its overexpression in transgenic S. miltiorrhiza improves tolerance to excess salt and drought conditions.

Keywords

LEA Salvia miltiorrhiza Abiotic stress Escherichia coli 

Notes

Acknowledgments

This work benefited from financial support from the Natural Science Foundation of Shaanxi Province, China (2014JQ3107) and the Fundamental Research Funds for the Central Universities (GK201302043).

Compliance with Ethical Standard

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Huaiqin Wang
    • 1
  • Yucui Wu
    • 1
  • Xinbing Yang
    • 1
  • Xiaorong Guo
    • 1
  • Xiaoyan Cao
    • 1
    Email author
  1. 1.Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of ChinaShaanxi Normal UniversityXi’anChina

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