Skip to main content

Advertisement

Springer Nature Link
Log in

Effects of fusaric acid treatment on the protocorm-like bodies of Dendrobium sonia-28

  • Original Article
  • Published:
Protoplasma Aims and scope Submit manuscript

Abstract

Dendrobium sonia-28 is a popular orchid hybrid due to its flowering recurrence and dense inflorescences. Unfortunately, it is being decimated by fungal diseases, especially those caused by Fusarium proliferatum. In this study, selection of F. proliferatum-tolerant protocorm-like bodies (PLBs) was carried out by assessing the effects of differing concentrations of fusaric acid (FA). PLBs were cultured on Murashige and Skoog (MS) medium supplemented with 0.05 to 0.2 millimolar (mM) concentrations of FA. Higher concentrations of FA increased mortality of PLBs and reduced their growth. The survival rate for 0.05 mM FA was 20 % but only 1 % at the highest dose of 0.2 mM. Additionally, two different size ranges of PLBs were investigated, and growth increased more at lower FA concentrations for larger PLBs, whilst the growth rate of smaller PLBs was inhibited at an FA concentration of 0.2 mM. Histological examination using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses disclosed severe cell wall and organelle damage, as well as stomatal closure in PLBs treated with the high FA concentrations. Reductions in plantlet growth were much greater at the highest concentrations of FA. Some randomly amplified polymorphic DNA (RAPD) markers clearly discriminated between selected and non-selected variants of Dendrobium sonia-28, showing different banding patterns for each FA concentration and specific bands for selected and control plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

Abbreviations

MS:

Murashige and Skoog

PLBs:

Protocorm-like bodies

FA:

Fusaric acid

mM:

Millimolar

RAPD:

Randomly amplified polymorphic DNA

References

  • Abbas HK, Paul RN, Boyette CD, Duke SO, Vesonder RF (1992) Physiological and ultrastructural effects of fumonisin on jims on weed leaves. Can J Bot 70:1824–1833

    Article  CAS  Google Scholar 

  • Achor DS, Nemec S, Baker RA (1993) Effects of Fusarium solani naphthazarin toxins on the cytology and ultrastructure of rough lemon seedlings. Mycopathologia 123(2):117–126

    Article  CAS  Google Scholar 

  • Allegre M, Daire X, Heloir MC, TrouvelotS ML, Adrian M, Pugin A (2007) Stomatal deregulation in Plasmopara viticola-infected grapevine leaves. New Phytol 173(4):832–840

    Article  CAS  PubMed  Google Scholar 

  • Asnita AH, Norzulaani K (2006) Direct regeneration and RAPD assessment of male inflorescence derived plants of Musa acuminata cv. Berangan. Asia Pac J Mol Biol Biotechnol 14(1):11–17

    Google Scholar 

  • Assmann SM (1993) Signal transduction in guard cells. Annu Rev Cell Biol 9(1):345–375

    Article  CAS  PubMed  Google Scholar 

  • Bacon CW, Porter JK, Norred WP, Leslie JF (1996) Production of fusaric acid by Fusarium species. Appl Environ Microbiol 62(11):4039–4043

    CAS  PubMed  PubMed Central  Google Scholar 

  • Behnke M (1979) Selection of potato callus for resistance to culture filtrates of Phytophthora infestans and regeneration of plants. Theor Appl Genet 55:69–71

    Article  CAS  PubMed  Google Scholar 

  • Bektas E, Cüce M, Sökmen A (2013) In vitro germination, protocorm formation, and plantlet development of Orchis coriophora (Orchidaceae), a naturally growing orchid species in Turkey. Turk J Bot 37(2):336–342

    CAS  Google Scholar 

  • Blatt MR, Grabov A, Brearley J, Hammond- Kosack K, Jones JD (1999) K+ channels of cf-9 transgenic tobacco guard cells as targets for Cladosporium fulvum Avr9 elicitor-dependent signal transduction. Plant J 19(4):453–462

    Article  CAS  PubMed  Google Scholar 

  • Bouizgarne B, Brault M, Pennarun AM, Rona JP, Ouhdouch Y, El Hadrami I, Bouteau F (2004) Electrophysiological responses to fusaric acid of root hairs from seedlings of date palm‐susceptible and‐resistant to Fusarium oxysporum f. sp. albedinis. J Phytopathol 152(6):321–324

    Article  CAS  Google Scholar 

  • Bouizgarne B, El-Maarouf-Bouteau H, Madiona K, Biligui B, Monestiez M, Pennarun AM, Amiar Z, Rona JP, Ouhdouch Y, El Hadrami I, Bouteau F (2006) A putative role for fusaric acid in biocontrol of the parasitic angiosperm Orobanche ramose. Mol Plant Microbe Interact 19(5):550–556

    Article  CAS  PubMed  Google Scholar 

  • Chaerle L, De Boever F, Montagu MV, Straeten D (2001) Thermographic visualization of cell death in tobacco and Arabidopsis. Plant Cell Environ 24(1):15–25

    Article  Google Scholar 

  • Chawla HS, Wenzel G (1987) In vitro selection for fusaric acid resistant barley plants. Plant Breed 99(2):159–163

    Article  Google Scholar 

  • Chen YH, Tsai YJ, Huang JZ, Chen FC (2005) Transcription analysis of peloric mutants of Phalaenopsis orchids derived from tissue culture. Cell Res 15(8):639–657

    Article  CAS  PubMed  Google Scholar 

  • Curir P, Guglieri L, Dolci M, Capponil A, Aurino G (2000) Fusaric acid production by Fusarium oxysporum f.sp. lilii and its role in the lily basal rot disease. Eur J Plant Pathol 106(9):849–856

    Article  CAS  Google Scholar 

  • D’Mello JPF, Macdonald AMC (1998) Fungal toxins as disease elicitors. In: Rose J (ed) Environmental toxicology: current developments. Gordon and Breach, Amsterdam, pp 253–289

    Google Scholar 

  • Damayanti F (2002) Seleksi in vitro untuk ketahanan terhadap penyakit layu Fusarium pada Tanaman Abaka (Musa textilis Nee.). [Tesis]. Bogor: Program Pascasarjana IPB Indonesia

  • Dehgahi R, Latiffah Z, Joniyas A, Subramaniam S (2014) Fusarium proliferatum culture filtrate sensitivity of Dendrobium sonia-28’s PLBs derived regenerated plantlets. Malays J Microbiol 10(4):241–248

    Google Scholar 

  • Diniz SPSS, Oliveira RC (2009) Effects of fusaric acid on Zea mays L. seedlings. Int J Bot 78:155–160

    Google Scholar 

  • Dong X, Ling N, Wang M, Shen Q, Gue S (2012) Fusaric acid is a crucial factor in the disturbance of leaf water imbalance in Fusarium-infected banana plants. Plant Physiol Biochem 60:171–179

    Article  CAS  PubMed  Google Scholar 

  • Gonzalez AI, Polanco C, Ruiz ML (2006) In vitro culture response of common bean explants to filtrate from Pseudomonas syringae pv. Phaseolicola and correlation with disease resistance. In Vitro Cell Dev Biol 42(2):160–164

    Article  Google Scholar 

  • Hamid K, Strange RN (2000) Phytotoxicity of solanapyrones A and B produced by the chickpea pathogen Ascochyta rabiei (Pass.) Labr. and the appparent metabolism of solanapyrone A by chickpea tissues. Physiol Mol Plant Pathol 56(6):235–244

    Article  CAS  Google Scholar 

  • Houri NM, Al-Zein MS, Westbury DB, Talhouk SN (2012) Reproductive success of the rare endemic Orchis galilaea (Orchidaceae) in Lebanon. Turk J Bot 36(6):677–682

    Google Scholar 

  • Işık K (2011) Rare and endemic species: why are they prone to extinction? Turk J Bot 35(4):411–417

    Google Scholar 

  • Ismaiel AA, Papenbrock J (2015) Mycotoxins: producing fungi and mechanisms of hypotoxicity. Agriculture 5(3):492–537

    Article  Google Scholar 

  • Jayasankar S, Li Z, Gray DJ (2000) In vitro selection of Vitis vinifera ‘Chardonnay’ with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase. Planta 211(2):200–208

    Article  CAS  PubMed  Google Scholar 

  • Julkifle AL, Poobathy R, Samian R, Subramaniam S (2012) Histological analyses of PLBs of Dendrobium sonia-28 in the recognition of cell competence for regeneration and Agrobacterium infection. Plant Omics J 5(6):514–517

    Google Scholar 

  • Kaeppler SM, Kaeppler HF, Rhee Y (2000) Epigenetic aspects of somaclonal variation in plants. Plant Mol Biol 43(2–3):179–188

    Article  CAS  PubMed  Google Scholar 

  • Khoddamzadeh AA, Sinniah UR, Kadir MA, Kadzimin SB, Mahmood M, Sreeramanan S (2013) Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb. f.) Christenson. Afr J Biotechnol 9(40):6632–6639

    Google Scholar 

  • Khosravi AR, Kadir MA, Kadzemin SB, Zaman FQ, De Silva AE (2009) RAPD analysis of colchicine induced variation of the Dendrobium Serdang beauty. Afr J Biotechnol 8(8):1455–1465

    CAS  Google Scholar 

  • Kumar S, Kumar S, Negi SP, Kanwar JK (2008) In vitro selection and regeneration of chrysanthemum (Dendranthema grandiflorum Tzelev) plants resistant to culture filtrate of Septoria obesa Syd. Vitro Cell Dev Biol 44(6):474–479

    Article  Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60(4):197–214

    Article  CAS  PubMed  Google Scholar 

  • Latiffah Z, Nur-Hayati MZ, Baharrudin S, Mazih Z (2009) Identification and pathogenicity of Fusarium species associated with root rot and stem rot of Dendrobium. Asian J Plant Pathol 3:14–21

    Article  Google Scholar 

  • Lee S, Choi H, Suh S, Doo IS, Oh KY, Choi EJ, Taylor ATS, Low PS, Lee Y (1999) Oligogalacturonic acid and chitosan reduce stomatal aperture by inducing the evolution of reactive oxygen species from guard cells of tomato and Commelina communis. Plant Physiol 121(1):147–152

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Manshardt R (2004) Crop improvement by conventional breeding or genetic engineering: how different are they? University of Hawaii. 3 p. (Biotechnology; BIO-5), Honolulu (HI)

    Google Scholar 

  • Mariska I, Lestari EG, Kosmiatin M, Roostika I (2005) Seleksi in vitro untuk mendapatkan tanaman pisang ambon yang tahan terhadap penyakit layu Fusarium. Laporan Rusnas Buah Tropika. Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian dan Institut Pertanian Bogor: BB-Biogen dan Pusat Kajian Buah Buahan Tropika. IPB Indonesia

  • Matsumoto K, Barbosa ML, Souza LAC, Teixeira JB (1995) Race 1 Fusarium wilt tolerance on banana plants selected by fusaric acid. Euphytica 84(1):67–71

    Article  Google Scholar 

  • Mott KA, Takemoto JY (1989) Syringomycin, a bacterial phytotoxin, closes stomata. Plant Physiol 90(4):1435–1439

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15(3):473–497

    Article  CAS  Google Scholar 

  • Nasir IA, Jamal A, Rahman Z, Husnain T (2012) Molecular analyses of gladiolus lines with improved resistance against Fusarium wilt. Pak J Bot 44(1):73–79

    CAS  Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A 76(10):5269–5273

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nicoletti B (2003) Number of orchids. In: Elert G (ed.) The physics factbook. An encyclopedia of scientific essays. Available at http://hypertextbook.com/facts/2003/BiancaNicoletti.Shtml

  • Peredo EL, Revilla MA, Arroyo-Garcıa R (2006) Assessment of genetic and epigenetic variation in hop plants regenerated from sequential subcultures of organogenic calli. J Plant Physiol 163(10):1071–1079

    Article  CAS  PubMed  Google Scholar 

  • Price Z, Dumortier F, MacDonald D, Mayes S (2002) Characterisation of copia-like retrotransposons in oil palm (Elaeis guineensis Jacq.). Theor Appl Genet 104(5):860–867

    Article  CAS  PubMed  Google Scholar 

  • Remotti PC, Loffler HJ, van Vloten-Doting L (1997) Selection of cell-lines and regeneration of plants resistant to fusaric acid from Gladiolus grandiflorus cv. ‘Peter Pears’. Euphytica 96(2):237–245

    Article  Google Scholar 

  • Saiprasad GVS, Polisetty R (2003) Propagation of three orchid genera using encapsulated protocorm-like bodies. Vitro Cell Dev Plants 39(1):42–48

    Article  Google Scholar 

  • Saxena G, Verma PC, Rahman LU, Banerjee S, Shukla RS, Kumar S (2008) Selection of leaf blight-resistant Pelargonium graveolens plants regenerated from callus resistant to a culture filtrate of Alternaria alternate. Crop Prot 27(3):558–565

    Article  CAS  Google Scholar 

  • Shahin EA, Spivey R (1986) A single dominant gene for Fusarium wilt resistance in protoplast-derived tomato plants. Theor Appl Genet 73(2):164–169

    Article  CAS  PubMed  Google Scholar 

  • Smith RCG, Heritage AD, Stapper M, Barrs HD (1986) Effect of stripe rust (Puccinia striiformis West.) and irrigation on the yield and foliage temperature of wheat. Field Crops Res 14:39–51

    Article  Google Scholar 

  • Sopalun K, Thammasiri K, Ishikawa K (2010) Micropropagation of the Thai orchid Grammatophyllum speciosum blume. Plant Cell Tiss Org 101(2):143–150

    Article  Google Scholar 

  • Souza IRP, Alves VMC, Parentoni SN, Oliveira ACD, Teixeira FF, Mac Adam JW, Purcino AÁC (2002) Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. Braz J Plant Physiol 14(3):219–224

    Article  Google Scholar 

  • Stankovic S, Levic J, Petrovic T, Logrieco A, Moretti A (2007) Pathogenicity and mycotoxin production by Fusarium proliferatum isolated from onion and garlic in Serbia. Eur J Plant Pathol 118(2):165–172

    Article  CAS  Google Scholar 

  • Švábová L, Lebeda A (2005) In vitro selection for improved plant resistance to toxin-producing pathogens. Phytopathol 153(1):52–64

    Article  Google Scholar 

  • Svábová L, Lebeda A, Kitner M, Sedlárová M, Petrivalsky M, Dostálová R, Ondrej M, Horacek J, Smykalova I, Griga M (2011) Comparison of the effects of Fusarium solani filtrates in vitro and in vivo on the morphological characteristics and peroxidase activity in Pea cultivars with different susceptibility. Plant Pathol 93(1):19–30

    Google Scholar 

  • Swarts ND, Dixon KW (2009) Terrestrial orchid conservation in the age of extinction. Ann Bot 104(3):543–556

    Article  PubMed  PubMed Central  Google Scholar 

  • Tripathi MK, Tiwari S, Khare UK (2008) In vitro selection for resistance against purple blotch disease of onion (Allium cepa L.) caused by Alternaria porri. Biotech 7(1):80–86

    Article  Google Scholar 

  • Wang M, Ling N, Dong X, Liu X, Shen Q, Guo S (2014) Effect of fusaric acid on the leaf physiology of cucumber seedlings. Eur J Plant Pathol 138(1):103–112

    Article  CAS  Google Scholar 

  • Wenzel G, Foroughi-Wehr B (1993) In vitro selection. In: Plant breeding. Springer Netherlands. pp. 353–370

  • William H, Outlaw Jr (2003) Integration of cellular and physiological functions of guard cells. Plant Sci 22(6):503–529

    Article  Google Scholar 

  • Willmer C, Fricker M (1996) Stomata, 2nd edn. Chapman & Hall, London, UK, p 375

    Book  Google Scholar 

  • Wu HS, Bao W, Liu DY, Ling N, Ying RR, Raza W, Shen QR (2008) Effect of fusaric acid on biomass and photosynthesis of watermelon seedlings leaves. Caryologia 61(3):258–268

    Article  CAS  Google Scholar 

  • Zeng W, Melotto M, He SY (2010) Plant stomata: a checkpoint of host immunity and pathogen virulence. Curr Opin Biotech 21(5):599–603

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

The authors are grateful to USM Research University 2012 (1001/PBIOLOGI/811211) for funding this project.

Author information

Authors and Affiliations

  1. School of Biological Sciences, Universiti Sains Malaysia, 11800, Georgetown, Penang, Malaysia

    Raheleh Dehgahi, Latiffah Zakaria, Alireza Joniyas & Sreeramanan Subramaniam

  2. Agrotechnology and Bioscience Division, Malaysian Nuclear Agency, 43000, Bangi, Kajang, Malaysia

    Azhar Mohamad

Authors
  1. Raheleh Dehgahi
    View author publications

    Search author on:PubMed Google Scholar

  2. Latiffah Zakaria
    View author publications

    Search author on:PubMed Google Scholar

  3. Azhar Mohamad
    View author publications

    Search author on:PubMed Google Scholar

  4. Alireza Joniyas
    View author publications

    Search author on:PubMed Google Scholar

  5. Sreeramanan Subramaniam
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Sreeramanan Subramaniam.

Additional information

Handling Editor: Hanns H. Kassemeyer

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dehgahi, R., Zakaria, L., Mohamad, A. et al. Effects of fusaric acid treatment on the protocorm-like bodies of Dendrobium sonia-28. Protoplasma 253, 1373–1383 (2016). https://doi.org/10.1007/s00709-015-0895-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00709-015-0895-1

Keywords