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Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress

  • Special Issue: Cell Biology in Agricultural and Food Science
  • Published:
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Abstract

Heat shock proteins (HSPs) exist extensively in eukaryotes and are conserved molecular chaperones with important contribution to plant’s survival under environmental stresses. Here, the cloning and characterization of one complementary DNA (cDNA) designated as BcHSP70 from young seedlings of Brassica campestris were reported in the present work. Bioinformatic analysis revealed that BcHSP70 belongs to the plant HSP gene family and had the closest relationship with HSP70-4 from Arabidopsis thaliana. Constitutive overexpression of BcHSP70 in tobacco obviously conferred tolerance to heat stress by affecting different plant physiological parameters. In our study, transgenic tobaccos exhibited higher chlorophyll content than wild-type control when exposed to heat stress. Superoxide dismutase (SOD) and peroxidase (POD) activities, which were helpful to decrease the damage to the membrane system, were significantly higher in transformants compared to wild-type lines. Meanwhile, lower comparative electrical conductivity and malondialdehyde (MDA) content and higher proline and soluble sugar accumulation were found in transgenic tobaccos than in wild-type lines. All these above results indicated that this isolated BcHSP70 cDNA owned the ability to improve the tolerance to heat stress in transgenic tobacco, which provides helpful information and good basement to culture new robust B. campestris variety resistant to high-temperature stress by molecular breeding in the future.

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Acknowledgments

This work was supported by the Shanghai Education Committee Fund (13ZZ104, J50401). Authors also wanted to acknowledge the important contribution from anonymous reviewers’ comments and revision for the paper.

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310018, People’s Republic of China

    Xiaorong Wang, Wei Zhou, Huizhong Wang & Guoyin Kai

  2. Institute of Plant Biotechnology, Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People’s Republic of China

    Xiaorong Wang, Bin Yan, Min Shi, David Zekria & Guoyin Kai

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  1. Xiaorong Wang
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  3. Min Shi
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Correspondence to Guoyin Kai.

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Handling Editor: Bhumi Nath Tripathi

Xiaorong Wang and Bin Yan are co-first authors.

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Fig. S1

Schematic representation of transformation plasmid (pCAMBIA2300 + -BcHSP70) (DOCX 659 kb)

Fig. S2

Representative PCR analysis for the presence of the BcHSP70 in the transgenic tobacco plants. M-size marker (2 kb, 1 kb, 750 bp, 500 bp, 250 bp, 100 bp) N-untransformed plant (negative control) and P- p2300 + -BcHSP70 (positive control). (DOC 61 kb)

Fig. S3

Growth state comparison of the transgenic tobacco plants with the wild type after exposure at 42 °C for 0 h and 3 h. (DOC 81 kb)

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Wang, X., Yan, B., Shi, M. et al. Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress. Protoplasma 253, 637–645 (2016). https://doi.org/10.1007/s00709-015-0867-5

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  • DOI: https://doi.org/10.1007/s00709-015-0867-5

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