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A putative soybean GmsSOS1 confers enhanced salt tolerance to transgenic Arabidopsis sos1-1 mutant

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Abstract

The cDNA of GmsSOS1, a putative plasma membrane Na+/H+ antiporter gene isolated from Glycine max, Glycine soja, and their hybrid, was constructed into plant expression vector pCAMBIA 1300 and then transformed with Agrobacterium tumefaciens under the control of CaMV 35S promoter to Arabidopsis thaliana wild-type (WT) and mutant (atsos1-1) plants. By hygromycin resistance detection and PCR analysis, transgenic plants (WT35S:GmsSOS1 and atsos1-1 35S:GmsSOS1) were obtained. Seed germination, seedling growth, and Na+ contents in roots and shoots were analytically compared among WT, atsos1-1 mutant, and their transgenic lines under salt stress. The results showed that when GmsSOS1 was integrated into the genome of A. thaliana, the inhibitions of salt stress on seed germination and seedling growth were all significantly improved, and enhanced salt tolerance was displayed, which may be attributed to the decrease of Na+ absorption in roots and transportation in shoots of the transgenic lines, especially for that of atsos1-1 mutant.

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References

  • Ashraf M, Akram NA (2009) Improving salinity tolerance of plants through conventional breeding and genetic engineering: an analytical comparison. Biotechnol Adv 27:744–752

    Article  CAS  PubMed  Google Scholar 

  • Chan C, Qi X, Li M-W, Wong F-L, Lam H-M (2012) Recent developments of genomic research in soybean. J Genet Genom 39:317–324

    Article  CAS  Google Scholar 

  • Chen W, Hou Z, Wu L, Liang Y, Wei C (2010) Effects of salinity and nitrogen on cotton growth in arid environment. Plant Soil 326:61–73

    Article  CAS  Google Scholar 

  • Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743

    Article  CAS  PubMed  Google Scholar 

  • Cosentino C, Fischer-Schliebs E, Bertl A, Thiel G, Homann U (2010) Na+/H+ antiporters are differentially regulated in response to NaCl stress in leaves and roots of Mesembryanthemum crystallinum. New Phytol 186:669–680

    Article  CAS  PubMed  Google Scholar 

  • Feki K, Quintero FJ, Pardo JM, Masmoudi K (2011) Regulation of durum wheat Na+/H+ exchanger TdSOS1 by phosphorylation. Plant Mol Biol 76:545–556

    Article  CAS  PubMed  Google Scholar 

  • Feki K, Quintero FJ, Khoudi H, Leidi EO, Masmoudi K, Pardo JM, Brini F (2014) A constitutively active form of a durum wheat Na+/H+ antiporter SOS1 confers high salt tolerance to transgenic Arabidopsis. Plant Cell Rep 33:277–288

    Article  CAS  PubMed  Google Scholar 

  • Gao S, Yuan L, Zhai H, Liu C, He S, Liu Q (2012) Overexpression of SOS genes enhanced salt tolerance in sweet potato. J Integr Agri 11:378–386

    Article  CAS  Google Scholar 

  • Hasegawa PM (2013) Sodium (Na+) homeostasis and salt tolerance of plants. Environ Exp Bot 92:19–31

    Article  CAS  Google Scholar 

  • Hassen A, Maher S, Cherif H (2014) Effect of salt stress (NaCl) on germination and early seedling parameters of three pepper cultivars (Capsicum annuum L.). J Stress Physiol Biochem 10:14–25

    Google Scholar 

  • Katiyar-Agarwal S, Zhu J, Kim K, Agarwal M, Fu X, Huang A, Zhu J-K (2006) The plasma membrane Na+/H+ antiporter SOS1 interacts with RCD1 and functions in oxidative stress tolerance in Arabidopsis. Proc Natl Acad Sci U S A 103:18816–18821

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kim MY, Lee S, Van K, Kim TH, Jeong SC, Choi IY, Kim DS, Lee YS, Park D, Ma J, Kim WY, Kim BC, Park S, Lee KA, Kim DH, Kim KH, Shin JH, Jang YE, Kim KD, Liu WX, Chaisan T, Kang YJ, Lee YH, Moon JK, Schmutz J, Jackson SA, Bhak J, Lee SH (2010) Whole-genome sequencing and intensive analysis of the undomesticated soybean (Glycine soja Sieb. and Zucc.) genome. Proc Natl Acad Sci U S A 107:22032–22037

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Lam H-M, Xu X, Liu X, Chen WB, Yang GH, Wong FL, Li MW, He WM, Qin N, Wang B, Li J, Jian M, Wang JA, Shao G, Wang J, Sun SSM, Zhang GY (2010) Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection. Nat Genet 42:1053–1059

    Article  CAS  PubMed  Google Scholar 

  • Li F (2012) Hybrid identification with SSR markers, analysis of salt tolerance, cloning and expression of GmsSOS1 in Glycine max, Glycine soja and their hybrid. Dissertation, Nanjing Agricultural University

  • Ma D-M, Xu W-R, Li H-W, Jin F-X, Guo L-N, Wang J, Dai H-J, Xu X (2014a) Co-expression of the Arabidopsis SOS genes enhances salt tolerance in transgenic tall fescue (Festuca arundinacea Schreb.). Protoplasma 251:219–231

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Maas EV, Hoffman GJ (1977) Crop salt tolerance—current assessment. J Irrig Drain Div 103(2):115–134

    Google Scholar 

  • Ma Q, Li Y-X, Yuan H-J, Hu J, Wei L, Bao A-K, Zhang J-L, Wang S-M (2014b) ZxSOS1 is essential for long-distance transport and spatial distribution of Na+ and K+ in the xerophyte Zygophyllum xanthoxylum. Plant Soil 374:661–676

    Article  CAS  Google Scholar 

  • Mahajan S, Pandey GK, Tuteja N (2008) Calcium- and salt-stress signaling in plants: shedding light on SOS pathway. Arch Biochem Biophys 471:146–158

    Article  CAS  PubMed  Google Scholar 

  • Martínez-Atienza J, Jiang X, Garciadeblas B, Mendoza I, Zhu J-K, Pardo JM, Quintero FJ (2007) Conservation of the salt overly sensitive pathway in rice. Plant Physiol 143:1001–1012

    Article  PubMed Central  PubMed  Google Scholar 

  • Munns R, Tester M (2008) Mechanisms of salinity tolerance. Ann Rev Plant Biol 59:651–681

    Article  CAS  Google Scholar 

  • Nawaz I, Iqbal M, Hakvoort HWJ, Bliek M, de Boer B, Schat H (2014) Expression levels and promoter activities of candidate salt tolerance genes in halophytic and glycophytic Brassicaceae. Environ Exp Bot 99:59–66

    Article  CAS  Google Scholar 

  • Oh DH, Leidi E, Zhang Q, Hwang SM, Li Y, Quintero FJ, Jiang X, D’Urzo MP, Lee SY, Zhao Y, Bahk JD, Bressan RA, Yun DJ, Pardo JM, Bohnert HJ (2009) Loss of halophytism by interference with SOS1 expression. Plant Physiol 151:210–222

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Oh DH, Lee SY, Bressan RA, Yun DJ, Bohnert HJ (2010) Intracellular consequences of SOS1 deficiency during salt stress. J Exp Bot 61:1205–1213

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Olías R, Eljakaoui Z, Li J, De Morales PAZA, Marín-Manzano MC, Pardo JM, Belver A (2009) The plasma membrane Na+/H+ antiporter SOS1 is essential for salt tolerance in tomato and affects the partitioning of Na+ between plant organs. Plant Cell Environ 32:904–916

    Article  PubMed  Google Scholar 

  • Qiu QS, Guo Y, Dietrich MA, Schumaker KS, Zhu JK (2002) Regulation of SOS1, a plasma membrane Na+/H+ exchanger in Arabidopsis thaliana by SOS2 and SOS3. Proc Natl Acad Sci U S A 99:8436–8441

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Quan R, Lin H, Mendoza I, Zhang Y, Cao W, Yang Y, Shang M, Chen S, Pardo JM, Guo Y (2007) SCABP8/CBL10, a putative calcium sensor, interacts with the protein kinase SOS2 to protect Arabidopsis shoots from salt stress. Plant Cell 19:1415–1431

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Quintero FJ, Ohta M, Shi HZ, Zhu JK, Pardo JM (2002) Reconstitution in yeast of the Arabidopsis SOS signaling pathway for Na+ homeostasis. Proc Natl Acad Sci U S A 99:9061–9066

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Quintero FJ, Martinez-Atienza J, Villalta I, Jiang X, Kim WY, Ali Z, Fujii H, Mendoza I, Yun DJ, Zhu JK, Pardo JM (2011) Activation of the plasma membrane Na+/H+ antiporter salt-overly-sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Proc Natl Acad Sci U S A 108:2611–2616

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Rozema J, Schat H (2013) Salt tolerance of halophytes, research questions reviewed in the perspective of saline agriculture. Environ Exp Bot 92:83–95

    Article  CAS  Google Scholar 

  • Schmutz J, Cannon SB, Schlueter J, Ma JX, Mitros T, Nelson W, Hyten DL, Song QJ, Thelen JJ, Cheng JL, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu SQ, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du JC, Tian ZX, Zhu LC, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183

    Article  CAS  PubMed  Google Scholar 

  • Shi HZ, Ishitani M, Kim C, Zhu JK (2000) The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter. Proc Natl Acad Sci U S A 97:6896–6901

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Shi HZ, Quintero FJ, Pardo JM, Zhu J-K (2002) The putative plasma membrane Na+/H+ antiporter SOS1 controls long distance Na+ transport in plants. Plant Cell 14(2):465–477

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Shi H, Lee BH, Wu SJ, Zhu JK (2003) Overexpressing of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana. Nat Biotechnol 21:81–85

    Article  CAS  PubMed  Google Scholar 

  • Türkana I, Demiral T (2009) Recent developments in understanding salinity tolerance. Environ Exp Bot 67:2–9

    Article  Google Scholar 

  • Wu S-J, Ding L, Zhu J-K (1996) SOS1, a genetic locus essential for salt tolerance and potassium acquisition. Plant Cell 8:617–627

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Xiao Y, Huang X, Shen Y, Huang Z (2013) A novel wheat α-amylase inhibitor gene, TaHPS, significantly improves the salt and drought tolerance of transgenic Arabidopsis. Physiol Plant 148:273–283

    Article  CAS  PubMed  Google Scholar 

  • Xu H, Jiang X, Zhan K, Cheng X, Chen X, Pardo JM, Cui D (2008) Functional characterization of a wheat plasma membrane Na+/H+ antiporter in yeast. Arc Biochem Biophys 47:8–15

    Article  Google Scholar 

  • Yang Q, Chen ZZ, Zhou X, Yin HB, Li X, Xin XF, Hong XH, Zhu JK, Gong ZZ (2009) Overexpression of SOS (salt overly sensitive) genes increases salt tolerance in transgenic Arabidopsis. Mol Plant 2:22–31

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Yue Y, Zhang M, Zhang J, Duan L, Li Z (2011) SOS1 gene overexpression increased salt tolerance in transgenic tobacco by maintaining a higher K+/Na+ ration. J Plant Physiol 169:255–261

    Article  PubMed  Google Scholar 

  • Zhang XK, Zhou QH, Cao JH, Yu BJ (2011) Differential Cl−/salt tolerance and NaCl-induced alternations of tissue and cellular ion fluxes in Glycine max, Glycine soja and their hybrid seedlings. J Agro Crop Sci 197:329–339

    Article  CAS  Google Scholar 

  • Zhu JK (2003) Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 6:441–445

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank greatly Associate Prof. Shi Huazhong, Department of Chemistry and Biochemistry, Texas Tech University, USA, for providing Arabidopsis seed materials. This work was supported by the National Transgenic Engineering Crops Breeding Special Funds of China (No. 2009ZX08004-008B) to BJ Yu.

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The authors declare that they have no conflict of interest.

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  1. Lab of Plant Stress Biology, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Wang-xing Nie, Lin Xu & Bing-jun Yu

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  1. Wang-xing Nie
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  2. Lin Xu
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Correspondence to Bing-jun Yu.

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Nie, Wx., Xu, L. & Yu, Bj. A putative soybean GmsSOS1 confers enhanced salt tolerance to transgenic Arabidopsis sos1-1 mutant. Protoplasma 252, 127–134 (2015). https://doi.org/10.1007/s00709-014-0663-7

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  • DOI: https://doi.org/10.1007/s00709-014-0663-7

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