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Exogenous EDDS modifies copper-induced various toxic responses in rice

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Abstract

Copper is a micronutrient required for living organisms, but is potentially toxic in excess. EDDS enhances the phytoextraction of many metals, but the underlying mechanism is fully unclear. Exposure of 200 μM Cu2+ for 3 days resulted in rice seedling growth inhibition, accompanied by a decrease in plasma membrane H+-ATPase activity, and an increase in relative electrolyte leakage ratios, indicating that maintaining of membrane structure integrity is crucial in acclimation of plants to heavy metal stress. In addition, the chlorophyll and carotenoid content was markedly decreased and the level of the mRNA of Cytochrome P450 gene, OsHMA9, the sulfate transporter gene, and the metallothionein-like protein gene was observed to increase in response to Cu stress. Cu treatment also induced a global epigenetic response which is associated with cell nucleus condensation. These physiological, genetic, and epigenetic responses of rice seedlings to excess copper were modified by the addition of EDDS, suggesting that the supply of EDDS in medium containing a high concentration of Cu ions could enhance plant tolerance potential to excess Cu toxicity through alleviating Cu-induced poisonous effects at various levels.

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Abbreviations

Cu:

Copper

DTT:

Dithiothreitol

EDDS:

Ethylenediamine-N,N′-disuccinic acid

PBS:

Phosphate-buffered saline

ROS:

Reactive oxygen species

H3K4me2:

Histone H3 di-methyl Lys4

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Acknowledgments

This work was supported by the NSFC (no. 31171186) and Hubei Province Natural Science Fund.

Conflict of interest

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “Exogenous EDDS modifies copper-induced various toxic responses in rice”.

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Authors and Affiliations

  1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Junjun Tan, Shibin He, Shihan Yan, Yingnan Li, Hui Li, Hao Zhang, Lin Zhao & Lijia Li

  2. State Key Laboratory of Cotton Biology, College of Life Sciences, Henan University, Kaifeng, 475004, People’s Republic of China

    Shibin He

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  1. Junjun Tan
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  2. Shibin He
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  7. Lin Zhao
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Correspondence to Lijia Li.

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Handling Editor: Liwen Jiang

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Supplemental Figure 1

Copper concentrations in shoots and roots. Bars represent standard errors of the mean. * denotes significant differences (p < 0.05) from the unexposed control using the Student’s t test. Rice roots showed significantly high Cu uptake following treatment with 200 μM of CuSO4 and Cu-EDDS, compared with the control, but the Cu concentration is still lower in shoots although exposure to 200 μM of CuSO4 and Cu-EDDS also resulted in a slight increase in copper concentration. (JPEG 823 kb)

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Tan, J., He, S., Yan, S. et al. Exogenous EDDS modifies copper-induced various toxic responses in rice. Protoplasma 251, 1213–1221 (2014). https://doi.org/10.1007/s00709-014-0628-x

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  • DOI: https://doi.org/10.1007/s00709-014-0628-x

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