, Volume 251, Issue 2, pp 363–372 | Cite as

Going "open" with Mesoscopy: a new dimension on multi-view imaging

  • Emilio Gualda
  • Nuno Moreno
  • Pavel Tomancak
  • Gabriel G. Martins
Special Issue: New/Emerging Techniques in Biological Microscopy


OpenSPIM and OpenSpinMicroscopy emerged as open access platforms for Light Sheet and Optical Projection Imaging, often called as optical mesoscopy techniques. Both projects can be easily reproduced using comprehensive online instructions that should foster the implementation and further development of optical imaging techniques with sample rotation control. This additional dimension in an open system offers the possibility to make multi-view microscopy easily modified and will complement the emerging commercial solutions. Furthermore, it is deeply based on other open platforms such as MicroManager and Arduino, enabling development of tailored setups for very specific biological questions. In our perspective, the open access principle of OpenSPIM and OpenSpinMicroscopy is a game-changer, helping the concepts of light sheet and optical projection tomography (OPT) to enter the mainstream of biological imaging.


Light sheet microscopy Optical projection tomography Fluorescence imaging Microscopy Mesoscopy Open source 



Three dimensional


Four dimensional


Applied Scientific Instrumentation


Computer aided design


Charged coupled device


Carbon dioxide


Central processing unit


Computed tomography


Digital scanned light microscopy


European Molecular Biology Laboratory




Graphics processing unit




Inverted selective plane illumination microscopy


Karlsruhe Institute of Technology


Light-emitting diode


Multidirectional selective plane illumination microscopy


Numerical aperture


Orthogonal-plane fluorescence optical sectioning


Optical projection tomography


Photon multiplier tube


Rapid automated modular microscope


Scientific complementary metal–oxide semiconductor


Structured illumination microscopy


Selective plane illumination microscopy





E.J.G. acknowledges support from the Fundação para a Ciência e a Tecnologia grant SFRH/BPD/80717/2011. GGM acknowledges the support of the EMBO practical course on 3D Developmental Imaging, and of the Microscopy Unit of Faculdade de Ciências, University of Lisbon. The Ditassa burchelli flower was kindly provided by Prof. Lia Ascensão.


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Emilio Gualda
    • 1
  • Nuno Moreno
    • 1
  • Pavel Tomancak
    • 2
  • Gabriel G. Martins
    • 1
    • 3
  1. 1.Instituto Gulbenkian de CiênciaOeirasPortugal
  2. 2.Max Planck Institute of Molecular Cell Biology and GeneticsDresdenGermany
  3. 3.Centro de Biologia AmbientalFaculdade de Ciencias da Universidade de LisboaCampo GrandePortugal

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