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Tissue-specific expression and functional role of dehydrins in heat tolerance of sugarcane (Saccharum officinarum)

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Abstract

Studies on the functional roles of dehydrins (DHNs) in heat tolerance of plants are scarce. This study was conducted to immunohistolocalize DHNs in leaves of heat-tolerant (CP-4333) and heat-sensitive (HSF-240) sugarcane (Saccharum officinarum L.) clones at three phenological stages in order to elucidate their putative roles under heat stress. CP-4333 indicated greater amounts of heat-stable proteins than HSF-240 under heat stress. Western blotting revealed the expression of three DHNs in CP-4333 (13- and 15-kDa peptides at 48 h and an additional 18-kDa band at 72 h) and two (13 and 15 kDa at 48 h) in HSF-240 at formative stage; two DHNs in CP-4333 (20 and 25 kDa) and one in HSF-240 (20 kDa) at grand growth stage, while two DHNs in CP-4333 (20 and 22 kDa) and one in HSF-240 (20 kDa) at maturity stage. Tissue-specific immunohistolocalization showed that DHNs were expressed in stele particularly the phloem and the cells intervening bundle sheath and vascular bundles. Furthermore, DHNs were also found scattered along the epidermal and parenchymatous cells. Recovery of sugarcane from heat stress manifested a gradual disappearance of DHNs in both the clones, being quicker in sensitive clone (HSF-240). Results suggested specific implications for DHNs synthesis. Their synthesis in epidermis appears to protect the mesophyll tissues from heat injury. When associated to vascular tissue, they tend to ensure the normal photoassimilate loading into the sieve element–companion cell complex. DHNs diminution during recovery suggested that their expression was transitory. However, prolonged retention of DHNs by tolerant clone appears to be an adaptive advantage of sugarcane to withstand heat stress.

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Acknowledgments

This study was financially supported by the Higher Education Commission of Pakistan. We highly appreciate the microtomy facility provided by Prof. Zargham and Prof. Ahrar, Univ. Agric., Faisalabad, Pakistan and the DHNs antibodies gift from Prof. Tim Close, UCR, USA. This paper is part of the PhD. thesis of the first author.

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There is no conflict of interest regarding this paper.

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Authors and Affiliations

  1. Khan Institute of Biotechnology and Genetic Engineering, University of Karachi, Karachi, Pakistan

    Saddia Galani

  2. Department of Botany, University of Agriculture, Faisalabad, Pakistan

    Abdul Wahid

  3. Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan

    Muhammad Arshad

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  1. Saddia Galani
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  2. Abdul Wahid
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  3. Muhammad Arshad
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Correspondence to Abdul Wahid.

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Handling Editor: Bhumi Nath Tripathi

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Galani, S., Wahid, A. & Arshad, M. Tissue-specific expression and functional role of dehydrins in heat tolerance of sugarcane (Saccharum officinarum). Protoplasma 250, 577–583 (2013). https://doi.org/10.1007/s00709-012-0443-1

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  • DOI: https://doi.org/10.1007/s00709-012-0443-1

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