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Organization of actin cytoskeleton during meiosis I in a wheat thermo-sensitive genic male sterile line

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Abstract

BS366 is a thermo-sensitive male sterile line of wheat (Triticum aestivum L.) for two-line hybrid breeding, which exhibits aberrant meiotic cytokinesis under low temperature. Through transcriptome analysis, a possible regulatory role for plant actin cytoskeleton was suggested. However, the organization of actin cytoskeleton in meiosis has been poorly understood so far. Here, fixed microsporocytes during meiosis were labeled with tetramethylrhodamine isothiocyanate–phalloidin and 4′,6-diamidino-2-phenylindole. Quantities of fluorescent micrographs were captured using a confocal microscope, including the transient state from metaphase to telophase. We observed that actin filaments were abundant in typical kariokinetic spindle, central spindle (parallel microtubules or actin fibers between two separated chromosomes in anaphase), and phragmoplast. Interestingly, we identified the Chinese lantern-shaped actin phragmoplast in wheat meiosis for the first time. Under low temperature, phragmoplast actin filaments were chaotic and normal cell plate failed to form. These data provide new insights into the organization of actin filaments during male meiosis of plant and support a role of actin cytoskeleton in bringing about thermo-sensitive male sterility in wheat.

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Acknowledgments

This work was supported by grants from the Beijing Natural Science Foundation (no.5091001), the National Natural Science Foundation of China (31171172), the National High Technology Research and Development Program of China (863 Program, no. 2011AA10A106), the Special Project for Innovation Ability Construction of BAAFS (KJCX201101007), the Beijing Innovational Platform Item for Foundational Research of Agricultural Breeding (nos. D08070500690801 and D111100001311002), and the China Agriculture Research System (no. CARS-3-1-4), respectively.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097, China

    Chenguang Xu, Zetao Liu, Liping Zhang, Changping Zhao, Shaohua Yuan & Fengting Zhang

  2. College of Life Sciences, Capital Normal University, Beijing, 100048, China

    Chenguang Xu, Zetao Liu, Liping Zhang & Changping Zhao

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  1. Chenguang Xu
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  2. Zetao Liu
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Correspondence to Liping Zhang or Changping Zhao.

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Handling Editor: Peter Nick

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Xu, C., Liu, Z., Zhang, L. et al. Organization of actin cytoskeleton during meiosis I in a wheat thermo-sensitive genic male sterile line. Protoplasma 250, 415–422 (2013). https://doi.org/10.1007/s00709-012-0386-6

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