Protoplasma

, Volume 249, Issue 1, pp 139–153 | Cite as

Cumulative effect of nitrogen and sulphur on Brassica juncea L. genotypes under NaCl stress

  • Manzer H. Siddiqui
  • Firoz Mohammad
  • M. Masrooor A. Khan
  • Mohamed H. Al-Whaibi
Original Article
First Online:
Received:
Accepted:

Abstract

In the present study, N and S assimilation, antioxidant enzymes activity, and yield were studied in N and S-treated plants of Brassica juncea (L.) Czern. & Coss. (cvs. Chuutki and Radha) under salt stress. The treatments were given as follows: (1) NaCl90 mM+N0S0 mg kg-1 sand (control), (2) NaCl90 mM+N60S0 mg kg-1 sand, (3) NaCl90 mM+N60S20 mg kg-1 sand, (4) NaCl90 mM+N60S40 mg kg-1 sand, and (5) NaCl90 mM+N60S60 mg kg-1 sand. The combined application of N (60 mg kg−1 sand) and S (40 mg kg−1 sand) proved beneficial in alleviating the adverse effect of salt stress on growth attributes (shoot length plant−1, fresh weight plant−1, dry weight plant−1, and area leaf−1), physio-biochemical parameters (carbonic anhydrase activity, total chlorophyll, adenosine triphosphate-sulphurylase activity, leaf N, K and Na content, K/Na ratio, activity of nitrate reductase, nitrite reductase, glutamine synthetase, glutamate synthase, catalase, superoxide dismutase, ascorbate peroxidase and glutathione reductase, and content of glutathione and ascorbate), and yield attributes (pods plant−1, seeds pod−1, and seed yield plant−1). Therefore, it is concluded that combined application of N and S induced the physiological and biochemical mechanisms of Brassica. The stimulation of antioxidant enzymes activity and its synergy with N and S assimilation may be one of the important mechanisms that help the plants to tolerate the salinity stress and resulted in an improved yield.

Keywords

Antioxidants Enzyme activities Osmoprotectants Salt stress N assimilation 

Notes

Acknowledgment

We thank anonymous reviewers for their valuable suggestions and critical reading of the manuscript and Professor Adel Salah Abdul-Jabbar (director of Attracting Outstanding Faculty and Researchers Program) for providing the opportunity to work in the Department of Botany and Microbiology, King Saud University, Saudi Arabia.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Manzer H. Siddiqui
    • 1
  • Firoz Mohammad
    • 2
  • M. Masrooor A. Khan
    • 2
  • Mohamed H. Al-Whaibi
    • 1
  1. 1.Department of Botany and Microbiology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Plant Physiology Section, Department of BotanyAligarh Muslim UniversityAligarhIndia

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