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Cytoplasmic compartmental response to local mechanical stimulation of internal tissue cells

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Summary.

A convenient experimental system was established to test how cells derived from higher-plant internal tissues respond to mechanical stimulation. Short-term culture of tobacco ovules in vitro led to the generation of bar-shaped cells from the parenchyma tissue of the ovule funicle. These cells are still connected to the mother tissue and are almost undifferentiated. The cells are translucent, and one end protrudes from the funicle, making them easy to manipulate and observe. Mechanical stimulation tests performed on these cells indicated that the cells are less sensitive to mechanical stimulation than epidermal hair cells but still possess the ability to respond to stimulation. Interestingly, the cells showed a cytoplasmic compartmental response to the stimulation. The nucleus, some plastids, and mitochondria were organized into a responsive unit that moved in unison to the stimulated sites, whereas most of the other organelles were not notably influenced by the stimulation. This suggests that the cytoplasm is highly organized and functionally divided in response to environmental stimulation.

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Abbreviations

DAPI:

4′,6-diamidino-2-phenylindole

NAO:

nucleus-associated organelles

RU:

responsive unit

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Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for the Development of Biology, College of Life Science, Wuhan University, Wuhan

    Liang-Huan Qu & Meng-Xiang Sun

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  1. Liang-Huan Qu
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  2. Meng-Xiang Sun
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Correspondence to Meng-Xiang Sun.

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Correspondence: Meng-Xiang Sun, Key Laboratory of the Ministry of Education for the Development of Biology, College of Life Science, Wuhan University, Wuhan 430072, People’s Republic of China.

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Qu, LH., Sun, MX. Cytoplasmic compartmental response to local mechanical stimulation of internal tissue cells. Protoplasma 233, 51–59 (2008). https://doi.org/10.1007/s00709-008-0304-0

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