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The effect of bupivacaine·HCl on the physical properties of neuronal membranes

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Abstract

Fluorescent probe techniques were used to evaluate the effect of bupivacaine·HCl on the physical properties (transbilayer asymmetric lateral and rotational mobilities, annular lipid fluidity and protein distribution) of synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortex. An experimental procedure was used based on selective quenching of both 1,3-di(1-pyrenyl)propane (Py-3-Py) and 1,6-diphenyl-1,3,5-hexatriene (DPH) by trinitrophenyl groups, and radiationless energy transfer (RET) from the tryptophans of membrane proteins to Py-3-Py. Bupivacaine·HCl increased the bulk lateral and rotational mobilities, and annular lipid fluidity in SPMVs lipid bilayers, and had a greater fluidizing effect on the inner monolayer than that of the outer monolayer. The magnitude of increasing effect on annular lipid fluidity in SPMVs lipid bilayer induced by bupivacaine·HCl was significantly far greater than magnitude of increasing effect of the drug on the lateral and rotational mobilities of bulk SPMVs lipid bilayer. It also caused membrane proteins to cluster. These effects of bupivacaine·HCl on neuronal membranes may be responsible for some, though not all, of the local anesthetic actions of bupivacaine·HCl.

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Abbreviations

BSA:

bovine serum albumin

DPH:

1,6-diphenyl-1,3,5-hexatriene

Hepes:

N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid

Py-3-Py:

1,3-di(1-pyrenyl)propane

RET:

radiationless energy transfer

SPMVs:

synaptosomal plasma membrane vesicles

TNBS:

2,4,6-trinitrobenzenesulfonic acid

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Authors and Affiliations

  1. Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan, 602-739, South Korea

    K. I. Koo, J. H. Bae, C. H. Lee, C. D. Yoon, J. H. Pyun, H. O. Jang & I. Yun

  2. Department of Oral and Maxillofacial Surgery and Clinical Pharmacology, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan, South Korea

    S. H. Shin

  3. Department of Prosthodontics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan, South Korea

    Y. C. Jeon

  4. Department of Oral Physiology and Molecular Biology, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan, South Korea

    M. K. Bae

  5. VA Medical Center Geriatric Research, Education & Clinical Center, and Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, MN, 55417, USA

    W. G. Wood

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  1. K. I. Koo
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  2. J. H. Bae
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Correspondence to I. Yun.

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Koo, K.I., Bae, J.H., Lee, C.H. et al. The effect of bupivacaine·HCl on the physical properties of neuronal membranes. Protoplasma 234, 3–12 (2008). https://doi.org/10.1007/s00709-008-0017-4

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  • DOI: https://doi.org/10.1007/s00709-008-0017-4

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