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Exposure of Vicia faba and Pisum sativum to copper-induced genotoxicity

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Abstract

The potential genotoxicity of Cu2+ was investigated in Vicia faba and Pisum sativum seedlings in hydroponic culture conditions. Cu2+ caused a dose-dependent increase in micronuclei frequencies in both plant models. Cytological analysis of root tips cells showed clastogenic and aneugenic effects of this heavy metal on V. faba root meristems. Cu2+ induced chromosomal alterations at the lowest concentration used (2.5 mM) when incubated for 42 h, indicating the potent mutagenic effect of this ion. A spectrum of chromosomal abnormalities was observed in V. faba root meristems, illustrating the genotoxic events leading to micronuclei formation.

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Abbreviations

MCN:

micronuclei

MH:

maleic hydrazide

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Acknowledgment

This work was supported by a grant from ADEME “Bioindicateurs de Qualité des Sols” (PNETOX 2003).

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Authors and Affiliations

  1. Laboratoire de Physiologie et Génétique Végétales, EA ERTAC, Université Blaise Pascal-Clermont II, Campus Universitaire des Cézeaux, 24, Avenue des Landais, 63177, Aubière, France

    D. Souguir, G. Ledoigt & P. Goupil

  2. Laboratoire de Physiologie et Génétique des Plantes à Intérêt Agronomique, Faculté des Sciences de Bizerte, 7021, Jarzouna, Tunisia

    D. Souguir & E. Ferjani

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  1. D. Souguir
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  2. E. Ferjani
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  3. G. Ledoigt
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  4. P. Goupil
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Correspondence to P. Goupil.

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Souguir, D., Ferjani, E., Ledoigt, G. et al. Exposure of Vicia faba and Pisum sativum to copper-induced genotoxicity. Protoplasma 233, 203–207 (2008). https://doi.org/10.1007/s00709-008-0004-9

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  • DOI: https://doi.org/10.1007/s00709-008-0004-9

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