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Additional nucleoli and NOR activity during meiotic prophase I in larch (Larix decidua Mill.)

Protoplasma volume 232, pages 109–120 (2007)Cite this article

Summary.

Transcriptional activity was investigated in successive stages of prophase I (male meiosis) of larch meiocytes. Br-UTP incorporated into RNA was detected by light and electron microscopy. Two peaks of RNA synthesis were identified in the nucleolus. The first occurred during the zygotene–pachytene stage and the second (not previously described in plant meiocytes) in the diplotene. These processes correlated with a considerable increase in nucleolus volume during these periods. At the end of the zygotene, several perinucleolar structures lying close to each other and containing rRNA, argyrophilic proteins, U3 small nucleolar RNA, and fibrillarin were observed. The occurrence of newly formed RNA was also observed in these structures. This suggests that the observed perinucleolar structures correspond to the additional nucleoli known from animals.

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  1. Department of Cell Biology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruń

    D. J. Smoliński, J. Niedojadło & A. Górska-Brylass

  2. School of Biological Sciences, University of Portsmouth, Portsmouth

    A. Noble

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  1. D. J. Smoliński
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  2. J. Niedojadło
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  3. A. Noble
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  4. A. Górska-Brylass
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Correspondence to D. J. Smoliński.

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Correspondence: D. J. Smoliński, Department of Cell Biology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100 Toruń, Poland.

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Smoliński, D., Niedojadło, J., Noble, A. et al. Additional nucleoli and NOR activity during meiotic prophase I in larch (Larix decidua Mill.). Protoplasma 232, 109–120 (2007). https://doi.org/10.1007/s00709-007-0270-y

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  • DOI: https://doi.org/10.1007/s00709-007-0270-y

  • Keywords: Larix decidua; rRNA; Meiotic prophase I; Small nucleolar RNA; Fibrillarin; Nucleologenesis.